Recombinant
RabMAb

Anti-CHMP2B antibody [EPR10807(B)] (ab157208)

Overview

  • Product name
    Anti-CHMP2B antibody [EPR10807(B)]
    See all CHMP2B primary antibodies
  • Description
    Rabbit monoclonal [EPR10807(B)] to CHMP2B
  • Tested applications
    Suitable for: WB, ICC/IF, IP, Flow Cytmore details
    Unsuitable for: IHC-P
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to residues in Human CHMP2B (Uniprot: Q9UQN3)

  • Positive control
    • HepG2, A431 and 293T cell lysates; fetal brain lysate; HepG2 and 293T cells.
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab157208 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 24 kDa.
ICC/IF 1/250 - 1/500.
IP 1/10 - 1/100.
Flow Cyt 1/10 - 1/100. ab172730-Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
  • Application notes
    Is unsuitable for IHC-P.
  • Target

    • Function
      Probable core component of the endosomal sorting required for transport complex III (ESCRT-III) which is involved in multivesicular bodies (MVBs) formation and sorting of endosomal cargo proteins into MVBs. MVBs contain intraluminal vesicles (ILVs) that are generated by invagination and scission from the limiting membrane of the endosome and mostly are delivered to lysosomes enabling degradation of membrane proteins, such as stimulated growth factor receptors, lysosomal enzymes and lipids. The MVB pathway appears to require the sequential function of ESCRT-O, -I,-II and -III complexes. ESCRT-III proteins mostly dissociate from the invaginating membrane before the ILV is released. The ESCRT machinery also functions in topologically equivalent membrane fission events, such as the terminal stages of cytokinesis and the budding of enveloped viruses (HIV-1 and other lentiviruses). ESCRT-III proteins are believed to mediate the necessary vesicle extrusion and/or membrane fission activities, possibly in conjunction with the AAA ATPase VPS4.
    • Tissue specificity
      Widely expressed. Expressed in brain, heart, skeletal muscle, spleen, kidney, liver, small intestine, pancreas, lung, placenta and leukocytes. In brain, it is expressed in cerebellum, cerebral cortex, medulla, spinal chord, occipital lobe, frontal lobe, temporal lobe and putamen.
    • Involvement in disease
      Defects in CHMP2B are the cause of frontotemporal dementia, chromosome 3-linked (FTD3) [MIM:600795]. FTD3 is characterized by an onset of dementia in the late 50's initially characterized by behavioral and personality changes including apathy, restlessness, disinhibition and hyperorality, progressing to stereotyped behaviors, non-fluent aphasia, mutism and dystonia, with a marked lack of insight. The brains of individuals with FTD3 have no distinctive neuropathological features. They show global cortical and central atrophy, but no beta-amyloid deposits.
    • Sequence similarities
      Belongs to the SNF7 family.
    • Domain
      The acidic C-terminus and the basic N-termminus are thought to render the protein in a closed, soluble and inactive conformation through an autoinhibitory intramolecular interaction. The open and active conformation, which enables membrane binding and oligomerization, is achieved by interaction with other cellular binding partners, probably including other ESCRT components.
    • Cellular localization
      Cytoplasm > cytosol. Late endosome membrane.
    • Information by UniProt
    • Database links
    • Alternative names
      • ALS17 antibody
      • Charged multivesicular body protein 2b antibody
      • CHM2B_HUMAN antibody
      • CHMP family, member 2B antibody
      • CHMP2.5 antibody
      • CHMP2b antibody
      • Chromatin modifying protein 2b antibody
      • Chromatin-modifying protein 2b antibody
      • DMT1 antibody
      • hVps2-2 antibody
      • Vacuolar protein sorting 2, yeast, homolog of, B antibody
      • Vacuolar protein sorting 2-2 antibody
      • Vacuolar protein sorting-associated protein 2-2 antibody
      • VPS2 homolog B antibody
      • Vps2-2 antibody
      • VPS2B antibody
      see all

    Images

    • Western blot analysis of Immunoprecipitation pellet from Human fetal brain lysate showing CHMP2B, using ab157208 at 1/10 dilution for IP
    • All lanes : Anti-CHMP2B antibody [EPR10807(B)] (ab157208) at 1/1000 dilution

      Lane 1 : fetal brain lysate
      Lane 2 : HepG2 cell lysate
      Lane 3 : A431 cell lysate
      Lane 4 : 293T cell lysate

      Lysates/proteins at 10 µg per lane.


      Predicted band size : 24 kDa
    • Immunofluorescence analysis of HepG2 cells, labeling CHMP2B using ab157208 at a 1/250 dilution.
    • Flow cytometry analysis of permeabilized 293T cells labeling CHMP2B (red), using ab157208 at a 1/10 dilution, and negative control cells probed with a Rabbit IgG (green)

    References

    ab157208 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab157208.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

    Sign up