Overview

  • Product nameAnti-CHMP4A antibody
    See all CHMP4A primary antibodies
  • Description
    Mouse polyclonal to CHMP4A
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Full length protein, corresponding to amino acids 1-223 of Human CHMP4A

  • Positive control
    • transfected 293T cells lysate

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferPreservative: None
    Constituents: 1X PBS, pH 7.2
  • Concentration information loading...
  • PurityProtein G purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab67058 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Predicted molecular weight: 25 kDa. Use BSA for blocking (see Abreview)

Target

  • RelevanceComponent of the ESCRT-III complex, which is required for multivesicular bodies (MVBs) formation and sorting of endosomal cargo proteins into MVBs. The MVB pathway mediates delivery of transmembrane proteins into the lumen of the lysosome for degradation. The ESCRT-III complex is probably involved in the concentration of MVB cargo. In the ESCRT-III complex, it probably serves as an acceptor for ESCRT-I complex on endosomal membranes. In case of infection, the HIV-1 virus takes advantage of the ESCRT-III complex for budding and exocytic cargos of viral proteins, via the association of CHMP4 proteins with PDCD6IP/AIP1, a protein directly recruited by HIV-1 p6 protein that functions at sites of viral Gag assembly and budding.
  • Cellular localizationCytoplasmic: Cytoplasmic vesicle membrane. Membrane-associated - localizes to large vesicle-like structures.
  • Database links
  • Alternative names
    • C14orf123 antibody
    • Charged multivesicular body protein 4a antibody
    • Chromatin modifying protein 4a antibody
    • HSPC134 antibody
    • SHAX2 antibody
    see all

Anti-CHMP4A antibody images

  • All lanes : Anti-CHMP4A antibody (ab67058) at 1/500 dilution

    Lane 1 : CHMP4A transfected 293T cell lysate
    Lane 2 : Non-transfected 293T cell lysate

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/5000 dilution

    Predicted band size : 25 kDa
    Observed band size : 30 kDa (why is the actual band size different from the predicted?)

References for Anti-CHMP4A antibody (ab67058)

ab67058 has not yet been referenced specifically in any publications.

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (293T)
Loading amount 30000 cells
Specification 293T
Gel Running Conditions Reduced Denaturing (4-20% Tris-Glycine)
Blocking step BSA as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
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Submitted May 27 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"