Overview

  • Product name
  • Description
    Mouse polyclonal to CHMP4C
  • Host species
    Mouse
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow, Chimpanzee, Cynomolgus monkey, Rhesus monkey, Chinese hamster
  • Immunogen

    Full length protein corresponding to amino acids 1-233 of Human CHMP4C (NP_689497.1).

  • Positive control
    • CHMP4C transfected 293T cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab168205 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 26 kDa.

Target

  • Function
    Probable core component of the endosomal sorting required for transport complex III (ESCRT-III) which is involved in multivesicular bodies (MVBs) formation and sorting of endosomal cargo proteins into MVBs. MVBs contain intraluminal vesicles (ILVs) that are generated by invagination and scission from the limiting membrane of the endosome and mostly are delivered to lysosomes enabling degradation of membrane proteins, such as stimulated growth factor receptors, lysosomal enzymes and lipids. The MVB pathway appears to require the sequential function of ESCRT-O, -I,-II and -III complexes. ESCRT-III proteins mostly dissociate from the invaginating membrane before the ILV is released. The ESCRT machinery also functions in topologically equivalent membrane fission events, such as the terminal stages of cytokinesis and the budding of enveloped viruses (HIV-1 and other lentiviruses). ESCRT-III proteins are believed to mediate the necessary vesicle extrusion and/or membrane fission activities, possibly in conjunction with the AAA ATPase VPS4. Involved in HIV-1 p6- and p9-dependent virus release.
  • Tissue specificity
    Expressed in heart, spleen and kidney.
  • Sequence similarities
    Belongs to the SNF7 family.
  • Domain
    The acidic C-terminus and the basic N-termminus are thought to render the protein in a closed, soluble and inactive conformation through an autoinhibitory intramolecular interaction. The open and active conformation, which enables membrane binding and oligomerization, is achieved by interaction with other cellular binding partners, probably including other ESCRT components.
  • Cellular localization
    Cytoplasm > cytosol. Late endosome membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • Charged multivesicular body protein 4c antibody
    • CHM4C_HUMAN antibody
    • CHMP4c antibody
    • Chromatin-modifying protein 4c antibody
    • hSnf7-3 antibody
    • hVps32-3 antibody
    • SHAX3 antibody
    • SNF7 homolog associated with Alix 3 antibody
    • SNF7-3 antibody
    • Vacuolar protein sorting-associated protein 32-3 antibody
    • Vps32-3 antibody
    see all

Images

  • All lanes : Anti-CHMP4C antibody (ab168205) at 1 µg/ml

    Lane 1 : CHMP4C transfected 293T cell lysate

    Lane 2 : Non-transfected 293T cell lysate


    Lysates/proteins at 15 µl per lane.

    Secondary
    All lanes : Goat Anti-Mouse IgG (H&L)-HRP at 1/2500 dilution

    Developed using the ECL technique.

    Predicted band size: 26 kDa

References

ab168205 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (Colon carcinoma)
Permeabilization
No
Specification
Colon carcinoma
Fixative
Methanol
Username

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Verified customer

Submitted Mar 22 2016

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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