Overview

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferPreservative: 0.02% Sodium Azide
    Constituents: 0.5% BSA, Tris buffered saline. pH 7.3
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Purification notesPurified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab31282 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 3 µg/ml.
ICC/IF Use at an assay dependent dilution. PubMed: 18997780

Target

  • FunctionProbable peripherally associated component of the endosomal sorting required for transport complex III (ESCRT-III) which is involved in multivesicular bodies (MVBs) formation and sorting of endosomal cargo proteins into MVBs. MVBs contain intraluminal vesicles (ILVs) that are generated by invagination and scission from the limiting membrane of the endosome and mostly are delivered to lysosomes enabling degradation of membrane proteins, such as stimulated growth factor receptors, lysosomal enzymes and lipids. The MVB pathway appears to require the sequential function of ESCRT-O, -I,-II and -III complexes. ESCRT-III proteins mostly dissociate from the invaginating membrane before the ILV is released. The ESCRT machinery also functions in topologically equivalent membrane fission events, such as the terminal stages of cytokinesis and the budding of enveloped viruses (HIV-1 and other lentiviruses). ESCRT-III proteins are believed to mediate the necessary vesicle extrusion and/or membrane fission activities, possibly in conjunction with the AAA ATPase VPS4. Involved in HIV-1 p6- and p9-dependent virus release.
  • Sequence similaritiesBelongs to the SNF7 family.
  • Cellular localizationCytoplasm > cytosol. Endosome membrane. Localizes to the midbody of dividing cells. Localized in two distinct rings on either side of the Fleming body.
  • Information by UniProt
  • Database links
  • Alternative names
    • apoptosis-related protein PNAS-2 antibody
    • C9orf83 antibody
    • CGI 34 antibody
    • Charged multivesicular body protein 5 antibody
    • CHMP 5 antibody
    • CHMP family, member 5 antibody
    • chmp5 antibody
    • CHMP5_HUMAN antibody
    • Chromatin modifying protein 5 antibody
    • Chromatin-modifying protein 5 antibody
    • Chromosome 9 open reading frame 83 antibody
    • HGNC:26942 antibody
    • HSPC177 antibody
    • hVps60 antibody
    • PNAS 2 antibody
    • SNF7 domain containing protein 2 antibody
    • SNF7 domain-containing protein 2 antibody
    • SNF7DC2 antibody
    • Vacuolar protein sorting 60 antibody
    • Vacuolar protein sorting-associated protein 60 antibody
    • Vps60 antibody
    see all

Anti-CHMP5 antibody images

  • Anti-CHMP5 antibody (ab31282) at 1 µg/ml + Cell lysate prepared from human K562 cell line at 35 µg
    Developed using the ECL technique

References for Anti-CHMP5 antibody (ab31282)

This product has been referenced in:
  • Yang D  et al. Structural basis for midbody targeting of spastin by the ESCRT-III protein CHMP1B. Nat Struct Mol Biol 15:1278-86 (2008). ICC/IF ; Human . Read more (PubMed: 18997780) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"