Overview

  • Product nameAnti-CHMP6 antibody
    See all CHMP6 primary antibodies
  • Description
    Rabbit polyclonal to CHMP6
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Cow, Orangutan
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human CHMP6.

    (Peptide available as ab90851.)

  • Positive control
    • This antibody gave a positive signal in the following whole cell lysates: Hela; Jurkat; HepG2; HEK293; MCF7; JEG3; K562.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab76929 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 50 kDa (predicted molecular weight: 23 kDa).

Target

  • FunctionProbable core component of the endosomal sorting required for transport complex III (ESCRT-III) which is involved in multivesicular bodies (MVBs) formation and sorting of endosomal cargo proteins into MVBs. MVBs contain intraluminal vesicles (ILVs) that are generated by invagination and scission from the limiting membrane of the endosome and mostly are delivered to lysosomes enabling degradation of membrane proteins, such as stimulated growth factor receptors, lysosomal enzymes and lipids. The MVB pathway appears to require the sequential function of ESCRT-O, -I,-II and -III complexes. ESCRT-III proteins mostly dissociate from the invaginating membrane before the ILV is released. The ESCRT machinery also functions in topologically equivalent membrane fission events, such as the terminal stages of cytokinesis and the budding of enveloped viruses (HIV-1 and other lentiviruses). ESCRT-III proteins are believed to mediate the necessary vesicle extrusion and/or membrane fission activities, possibly in conjunction with the AAA ATPase VPS4. In the ESCRT-III complex, it probably serves as an acceptor for the ESCRT-II complex on endosomal membranes.
  • Tissue specificityUbiquitously expressed.
  • Sequence similaritiesBelongs to the SNF7 family.
  • DomainThe acidic C-terminus and the basic N-termminus are thought to render the protein in a closed, soluble and inactive conformation through an autoinhibitory intramolecular interaction. The open and active conformation, which enables membrane binding and oligomerization, is achieved by interaction with other cellular binding partners, probably including other ESCRT components.
  • Cellular localizationEndomembrane system. Endosome membrane. Late endosome membrane. Localizes to endosomal membranes.
  • Information by UniProt
  • Database links
  • Alternative names
    • Charged multivesicular body protein 6 antibody
    • chmp6 antibody
    • CHMP6_HUMAN antibody
    • Chromatin modifying protein 6 antibody
    • Chromatin-modifying protein 6 antibody
    • FLJ11749 antibody
    • hVps 20 antibody
    • hVps20 antibody
    • novel SNF7 protein antibody
    • OTTMUSP00000004363 antibody
    • RGD1565325 antibody
    • RP24 225K18.4 antibody
    • Vacuolar protein sorting associated protein 20 antibody
    • Vacuolar protein sorting-associated protein 20 antibody
    • Vps 20 antibody
    • Vps20 antibody
    see all

Anti-CHMP6 antibody images

  • All lanes : Anti-CHMP6 antibody (ab76929) at 1 µg/ml

    Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 2 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
    Lane 3 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
    Lane 4 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
    Lane 5 : MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate
    Lane 6 : JEG-3 (Human placental choriocarcinoma cell line) Whole Cell Lysate
    Lane 7 : K562 (Human erythromyeloblastoid leukemia cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 23 kDa
    Observed band size : 50 kDa (why is the actual band size different from the predicted?)


    Exposure time : 30 seconds

References for Anti-CHMP6 antibody (ab76929)

ab76929 has not yet been referenced specifically in any publications.

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (HeLa)
Specification HeLa
Fixative Paraformaldehyde
Permeabilization Yes - 0.5% Triton X100
Username

Dr. Kirk McManus

Verified customer

Submitted Aug 03 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"