Anti-Cholecystokinin 8 antibody [27F3..0,5D10] (ab37274)
Key features and details
- Mouse monoclonal [27F3..0,5D10] to Cholecystokinin 8
- Suitable for: IHC-FoFr, ELISA
- Reacts with: Rat
- Isotype: IgG2a
Overview
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Product name
Anti-Cholecystokinin 8 antibody [27F3..0,5D10] -
Description
Mouse monoclonal [27F3..0,5D10] to Cholecystokinin 8 -
Host species
Mouse -
Specificity
Reacts with CCK-8 and the other CCK analogs with identical C-terminal ends as well as Gastrin 17. No reactivity was observed with Human Heparin cofactor II. -
Tested applications
Suitable for: IHC-FoFr, ELISAmore details -
Species reactivity
Reacts with: Rat -
Immunogen
CCK-8 fragment 26-33 GGDRDY(SO3H)MGWMDF
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C long term. -
Storage buffer
pH: 7.40
Constituents: 2.9% Sodium chloride, 0.0268% PBS -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
27F3..0,5D10 -
Myeloma
x63-Ag8.653 -
Isotype
IgG2a -
Light chain type
kappa -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab37274 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-FoFr |
Use at an assay dependent concentration. PubMed: 22892424
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ELISA |
Use at an assay dependent concentration.
We recommend coating with CCK-8 and using the antibody in a competitive assay protocol. Increased reactivity with sulphated CCK-8 compared to non-sulphated CCK-8 has been observed. |
Notes |
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IHC-FoFr
Use at an assay dependent concentration. PubMed: 22892424 |
ELISA
Use at an assay dependent concentration. We recommend coating with CCK-8 and using the antibody in a competitive assay protocol. Increased reactivity with sulphated CCK-8 compared to non-sulphated CCK-8 has been observed. |
Target
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Cellular localization
Secreted -
Database links
- Entrez Gene: 25298 Rat
- SwissProt: P01355 Rat
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Alternative names
- CCK antibody
- CCK8 antibody
- Cholecystokinin 8 antibody
- Cholecystokinin antibody
Datasheets and documents
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Datasheet download
References (10)
ab37274 has been referenced in 10 publications.
- Rice HC et al. Contribution of GABAergic interneurons to amyloid-ß plaque pathology in an APP knock-in mouse model. Mol Neurodegener 15:3 (2020). PubMed: 31915042
- Boccalaro IL et al. Dopamine depletion induces neuron-specific alterations of GABAergic transmission in the mouse striatum. Eur J Neurosci 52:3353-3374 (2020). PubMed: 32599671
- Bülbül M et al. Acute restraint stress induces cholecystokinin release via enteric apelin. Neuropeptides 73:71-77 (2019). PubMed: 30503693
- Modi B et al. Possible Implication of the CA2 Hippocampal Circuit in Social Cognition Deficits Observed in the Neuroligin 3 Knock-Out Mouse, a Non-Syndromic Animal Model of Autism. Front Psychiatry 10:513 (2019). PubMed: 31379628
- Bülbül M et al. Central apelin administration and restraint stress induce hypothalamic cholecystokinin release via the APJ receptor. J Neuroendocrinol 30:e12635 (2018). PubMed: 30044523
- Packard AEB et al. Sucrose-induced plasticity in the basolateral amygdala in a 'comfort' feeding paradigm. Brain Struct Funct 222:4035-4050 (2017). PubMed: 28597100
- Harden SW & Frazier CJ Oxytocin depolarizes fast-spiking hilar interneurons and induces GABA release onto mossy cells of the rat dentate gyrus. Hippocampus 26:1124-39 (2016). PubMed: 27068005
- Früh S et al. Neuronal Dystroglycan Is Necessary for Formation and Maintenance of Functional CCK-Positive Basket Cell Terminals on Pyramidal Cells. J Neurosci 36:10296-10313 (2016). IHC ; Mouse . PubMed: 27707967
- Iijima T et al. Neuronal cell type-specific alternative splicing is regulated by the KH domain protein SLM1. J Cell Biol 204:331-42 (2014). PubMed: 24469635
- Thibault K et al. Activation-Dependent Subcellular Distribution Patterns of CB1 Cannabinoid Receptors in the Rat Forebrain. Cereb Cortex : (2012). WB ; Rat . PubMed: 22892424