Overview

  • Product nameAnti-Cholecystokinin 8 antibody
    See all Cholecystokinin 8 primary antibodies
  • Description
    Rabbit polyclonal to Cholecystokinin 8
  • Tested applicationsSuitable for: IHC-P, ICC/IF, WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide sulfated cholecystokinin (26-33) amide (sulfated Cholecystokinin 8).

  • Positive control
    • Stomach. This antibody gave a positive result when used in the following formaldehyde fixed cell lines: CaCO2

Properties

Applications

Our Abpromise guarantee covers the use of ab27441 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/200. Do not perform antigen retrieval. Incubate for 10 minutes at room temperature.
ICC/IF Use a concentration of 10 µg/ml.
WB Use at an assay dependent concentration. Predicted molecular weight: 12 kDa.

Target

Anti-Cholecystokinin 8 antibody images

  • ICC/IF image of ab27441 stained CaCO2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab27441 at 10µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • Ab27441, at a dilution of 1/200, staining Cholecystokinin 8 in formalin fixed, paraffin embedded stomach tissue by Immunohistochemistry.
  • Anti-Cholecystokinin 8 antibody (ab27441) at 1/25 dilution + Brain cell lysate

    Predicted band size : 12 kDa
    Observed band size : 12 kDa

References for Anti-Cholecystokinin 8 antibody (ab27441)

This product has been referenced in:
  • Ye DZ & Kaestner KH Foxa1 and Foxa2 control the differentiation of goblet and enteroendocrine L- and D-cells in mice. Gastroenterology 137:2052-62 (2009). IHC-P ; Mouse . Read more (PubMed: 19737569) »

See 1 Publication for this product

Product Wall

I just received some extra information from the originator of this product. They have confirmed that this antibody does not require antigen retrieval,and the recommended dilution is 1:200,10min at RT. However, these condition may not match with Ven...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"