Anti-Cholera Toxin antibody [B041M] (ab62970)


  • Product nameAnti-Cholera Toxin antibody [B041M]
    See all Cholera Toxin primary antibodies
  • Description
    Mouse monoclonal [B041M] to Cholera Toxin
  • Specificityab62970 reacts with cholera toxin beta subunit.
  • Tested applicationsSuitable for: ELISAmore details
  • Immunogen

    Purified beta subunit of Cholera Toxin.


  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage bufferPreservative: 0.1% Sodium Azide
    Constituents: PBS, pH 7.4
  • Concentration information loading...
  • PurityProtein A purified
  • Purification notes>90% pure (SDS-PAGE).
  • ClonalityMonoclonal
  • Clone numberB041M
  • MyelomaSp2/0
  • IsotypeIgG2a
  • Research areas


Our Abpromise guarantee covers the use of ab62970 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent dilution.


  • RelevanceThe holotoxin (choleragen) consists of a pentameric ring of B subunits whose central pore is occupied by the A subunit. The A subunit contains two chains, A1 and A2, linked by a disulfide bridge. The B subunit pentameric ring directs the A subunit to its target by binding to the GM1 gangliosides present on the surface of the intestinal epithelial cells. It can bind five GM1 gangliosides. It has no toxic activity by itself. After binding to gangliosides GM1 in lipid rafts, through the subunit B pentamer, the holotoxin and the gangliosides are internalized. The holotoxin remains bound to GM1 until arrival in the ER. The A subunit has previously been cleaved in the intestinal lumen but the A1 and A2 chains have remained associated. In the ER, the A subunit disulfide bridge is reduced, the A1 chain is unfolded by the PDI and disassembled from the rest of the toxin. Then, the membrane-associated ER oxidase ERO1 oxidizes PDI, which releases the unfolded A1 chain. The next step is the retrotranslocation of A1 into the cytosol. This might be mediated by the protein-conducting pore SEC61. Upon arrival in the cytosol, A1 refolds and avoids proteasome degradation. In one way or another, A1 finally reaches its target and induces toxicity.
  • Cellular localizationSecreted
  • Alternative names
    • ctxA antibody
    • Cholera enterotoxin B chain antibody
    • Cholera enterotoxin gamma chain antibody
    • Cholera enterotoxin subunit A antibody
    • Cholera enterotoxin subunit B antibody
    • Choleragenoid antibody
    • ctxA antibody
    • ctxB antibody
    • toxA antibody
    • toxB antibody
    see all

References for Anti-Cholera Toxin antibody [B041M] (ab62970)

ab62970 has not yet been referenced specifically in any publications.

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