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Abcam's Cholesterol/Cholesteryl Ester Assay Kit - Detection provides a simple method for sensitive quantification of free cholesterol, cholesteryl esters, or both using a colorimetric method. Most of the cholesterol in blood is in the form of cholesteryl ester. These esters can be hydrolyzed to free cholesterol under the appropriate conditions. In the assay, free cholesterol is oxidized by cholesterol dehydrogenase to generate NADH which reacts with a sensitive probe resulting in strong absorbance at 450 nm. The assay can detect free or total cholesterol depending upon whether esterase is utilized to hydrolyze cholesterol esters present. Cholesteryl ester can be determined by subtracting the value of free cholesterol from the total cholesterol (cholesterol plus cholesteryl esters). The probe in this kit is more stable, sensitive and specific. The assay can tolerate interferences from various samples significantly.
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Cholesterol is an essential molecule in all animal life. It has been involved in both normal development and diseases.
|Cholesterol Assay Buffer||WM||1 x 25ml|
|Cholesterol Standard||Yellow||1 x 100µl|
|Enzyme Mix (lyophilized)||Green||1 vial|
|Esterase (lyophilized)||Blue||1 vial|
|Substrate Mix (lyophilized)||Red||1 vial|
Our Abpromise guarantee covers the use of ab102515 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Functional Studies||Use at an assay dependent dilution.|