Anti-Choline Acetyltransferase antibody [1E6] (ab35948)

Overview

  • Product nameAnti-Choline Acetyltransferase antibody [1E6]
    See all Choline Acetyltransferase primary antibodies
  • Description
    Mouse monoclonal [1E6] to Choline Acetyltransferase
  • SpecificityThis antibody is specific for cholinergic neurons in the brain and central nervous system.
  • Tested applicationsSuitable for: IHC-FrFl, IHC-Frmore details
  • Species reactivity
    Reacts with: Rat, Chicken
  • Immunogen

    Full length native protein from rat brain.

Properties

Applications

Our Abpromise guarantee covers the use of ab35948 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-FrFl 1/250.
IHC-Fr 1/100 - 1/250.

Target

  • FunctionCatalyzes the reversible synthesis of acetylcholine (ACh) from acetyl CoA and choline at cholinergic synapses.
  • Involvement in diseaseMyasthenic syndrome, congenital, 6, presynaptic
  • Sequence similaritiesBelongs to the carnitine/choline acetyltransferase family.
  • Information by UniProt
  • Database links
  • Alternative names
    • Acetyl CoA choline O acetyltransferase antibody
    • Acetyl CoA:choline O acetyltransferase antibody
    • ChAT antibody
    • CHOACTase antibody
    • Choline acetylase antibody
    • choline acetyltransferase antibody
    • Choline O acetyltransferase antibody
    • Choline O-acetyltransferase antibody
    • CLAT_HUMAN antibody
    • CMS1A antibody
    • CMS1A2 antibody
    • EC 2.3.1.6 antibody
    • OTTHUMP00000019583 antibody
    • OTTHUMP00000019584 antibody
    see all

Anti-Choline Acetyltransferase antibody [1E6] images

  • ab35948 at a 1/250 dilution staining Choline Acetyltransferase in mouse brain tissue sections by Immunohistochemistry (free floating sections) incubated for 12 hours at +4°C. Secondary used at 1/250 monoclonal Goat anti-mouse conjugated to biotin.

    See Abreview

References for Anti-Choline Acetyltransferase antibody [1E6] (ab35948)

ab35948 has not yet been referenced specifically in any publications.

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The predicted molecular weight can be found on Uniprot website.

http://www.uniprot.org/uniprot/P28329

http://www.uniprot.org/uniprot/P32738

Please note the molecular weight given is pred...

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Unfortunately, we do not routinely determine the concentration of ascitic fluid formats such as the Anti-Choline Acetyltransferase antibody [1E6] (ab35948). However, according to o...

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Application Immunohistochemistry free floating
Sample Mouse Tissue sections (Brain)
Specification Brain
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Abcam user community

Verified customer

Submitted Dec 23 2009

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Rat Cell (Spinal cord motoneurons)
Specification Spinal cord motoneurons
Fixative Paraformaldehyde
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10
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Abcam user community

Verified customer

Submitted Jan 10 2007

Thank you for your review. I am sorry to hear that you have been having difficulties with this antibody. Following correspondence with the source of this antibody I can tell you that Choline Acetyltransferase antibody [1E6] (ab35948) has actually be...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"