Anti-Choline Acetyltransferase antibody (ab18736)
- Datasheet
- Specific References (7)
- Protocols
Overview
- Product nameAnti-Choline Acetyltransferase antibody
See all Choline Acetyltransferase primary antibodies - DescriptionSheep polyclonal to Choline Acetyltransferase
- Tested applicationsSuitable for: IHC-FoFr, IHC-P, WB, ICC/IFmore details
- Species reactivityReacts with: Mouse, Rat, Rabbit, Guinea pig, Human, Pig
Predicted to work with: Chicken
- Immunogen
Synthetic peptide:
GLFSSYRLPGHTQDTLVAQKSS
conjugated to KLH, corresponding to amino acids 167-188 of Pig Choline Acetyltransferase.
Properties
- FormLiquid
- Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Add glycerol to a final volume of 50% for extra stability and aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
- Storage bufferPreservative: None
Constituents: Whole serum - PurityWhole antiserum
- ClonalityPolyclonal
- IsotypeIgG
- Research areas
Applications
Our Abpromise guarantee covers the use of ab18736 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-FoFr | 1/1000. Use on perfusion fixed tissue (1% sodium Nitrite in 0.1M TBS, pH, 7.4 for 20 min then 4% formaldehyde containing 15% picric acid in 0.1M TBS,pH, 7.4 for 20min). Please note sections must be post fixed if the tissue is cut freshly. |
|
| IHC-P | Use at an assay dependent concentration. | |
| WB | Use at an assay dependent concentration. Predicted molecular weight: 83 kDa. | |
| ICC/IF | 1/100. PubMed: 18393628 |
Target
- FunctionCatalyzes the reversible synthesis of acetylcholine (ACh) from acetyl CoA and choline at cholinergic synapses.
- Involvement in diseaseMyasthenic syndrome, congenital, 6, presynaptic
- Sequence similaritiesBelongs to the carnitine/choline acetyltransferase family.
- Information by UniProt
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Database links
- Entrez Gene: 100718930 Guinea pig
- Entrez Gene: 1103 Human
- Entrez Gene: 12647 Mouse
- Entrez Gene: 396896 Pig
- Entrez Gene: 100342226 Rabbit
- Entrez Gene: 290567 Rat
- Omim: 118490 Human
- SwissProt: P28329 Human
see all -
Alternative names
- Acetyl CoA choline O acetyltransferase antibody
- Acetyl CoA:choline O acetyltransferase antibody
- Chat antibody
see all
Anti-Choline Acetyltransferase antibody images
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Immunohistochemistry (PFA perfusion fixed frozen sections) - Anti-Choline Acetyltransferase antibody (ab18736)This image is courtesy of an Abreview submitted by Carla Cabralab18736 staining Choline Acetyltransferase in mouse brain tissue sections by Immunohistochemistry (PFA perfusion fixed frozen sections). Tissue samples were fixed by perfusion with paraformaldehyde, permeablized with 0.3% Triton X-100 and blocked with 3% donkey serum in 0.3% Triton X-100/TBS for 1 hour at 21°C. The sample was incubated with primary antibody (1/1000 in 1% donkey serum + 0.3% Triton X-100/TBS) at 4°C for 24 hours. An Alexa Fluor® 594-conjugated donkey anti-sheep polyclonal (1/200) was used as the secondary antibody.
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Immunocytochemistry/ Immunofluorescence - Anti-Choline Acetyltransferase antibody (ab18736)Immunofluorescence of Rat Forebrain tissue labeling ChAT with ab18736 using a 1µg dilution.
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Immunohistochemistry - Anti-Choline Acetyltransferase antibody (ab18736)Immunohistochemistry of Free-floating Rat forebrain tissue labeling ChAT with ab18736 at 1µg dilution.
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Immunohistochemistry (PFA perfusion fixed frozen sections) - Choline Acetyltransferase antibody (ab18736)Immunohistochemical detection of ChAT in rat basal forebrain using Sheep anti ChAT (ab18736).
Rat was perfused with 1% sodium Nitrite in 0.1M TBS, pH, 7.4 for 20 min. and then fixed with 4% formaldehyde containing 15% picric acid in 0.1M TBS, pH, 7.4 for 30 min. The brain was then cryoprotected in 30% overnight and cut into 40 nm free-floating sections. Sections were washed 2x with TBS for 20 min. and passed through asending ethanol and finally transferred to 0.1 M TBS for rehydration for 1h.
References for Anti-Choline Acetyltransferase antibody (ab18736)
This product has been referenced in:
- Yu B et al. Mechanisms Underlying Footshock and Psychological Stress-Induced Abrupt Awakening From Posttraumatic "Nightmares". Int J Neuropsychopharmacol N/A:N/A (2015). Rat . Read more (PubMed: 26591007) »
- Hadzipasic M et al. Selective degeneration of a physiological subtype of spinal motor neuron in mice with SOD1-linked ALS. Proc Natl Acad Sci U S A 111:16883-8 (2014). IHC ; Mouse . Read more (PubMed: 25385594) »
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"