Anti-Choline Acetyltransferase antibody (ab18736)

ab18736 staining Choline Acetyltransferase in mouse brain tissue sections by Immunohistochemistry (PFA perfusion fixed frozen sections). Tissue samples were fixed by perfusion with paraformaldehyde, permeablized with 0.3% Triton X-100 and blocked with 3% donkey serum in 0.3% Triton X-100/TBS for 1 hour at 21°C. The sample was incubated with primary antibody (1/1000 in 1% donkey serum + 0.3% Triton X-100/TBS) at 4°C for 24 hours. An Alexa Fluor® 594-conjugated donkey anti-sheep polyclonal (1/200) was used as the secondary antibody.

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Immunofluorescence of Rat Forebrain tissue labeling ChAT with ab18736 using a 1µg dilution. 

Immunohistochemistry of Free-floating Rat forebrain tissue labeling ChAT with ab18736 at 1µg dilution.

Immunohistochemical detection of ChAT in rat basal forebrain using Sheep anti ChAT (ab18736).

Rat was perfused with 1% sodium Nitrite in 0.1M TBS, pH, 7.4 for 20 min. and then fixed with 4% formaldehyde containing 15% picric acid in 0.1M TBS, pH, 7.4 for 30 min. The brain was then cryoprotected in 30% overnight and cut into 40 nm free-floating sections. Sections were washed 2x with TBS for 20 min. and passed through asending ethanol and finally transferred to 0.1 M TBS for rehydration for 1h.