Anti-Choline Acetyltransferase antibody (ab34419)

Submit an Abreview Q&A (4)Specific References (1)

Overview

  • Product name
    Anti-Choline Acetyltransferase antibody
    See all Choline Acetyltransferase primary antibodies
  • Description
    Chicken polyclonal to Choline Acetyltransferase
  • Host species
    Chicken
  • Tested applications
    Suitable for: WB, ICC/IF, IHC-Fr, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide (Mouse)

  • Positive control
    • Mouse/rat brain.

Properties

Applications

Our Abpromise guarantee covers the use of ab34419 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/250 - 1/500. Predicted molecular weight: 83 kDa.
ICC/IF 1/100 - 1/200.
IHC-Fr 1/50.
IHC-P Use at an assay dependent dilution.

Target

Images

  • Immunocytochemistry/ Immunofluorescence - Anti-Choline Acetyltransferase antibody (ab34419)
    Immunocytochemistry/ Immunofluorescence - Anti-Choline Acetyltransferase antibody (ab34419)
    This image shows a section of a formalin-fixed paraffin-embedded adult mouse brain showing ChAT (red staining) in cholinergic neurons of the caudate-putamen nucleus. Antibody ab34419 at a dilution of 1/100.

References

This product has been referenced in:
  • Bricker-Anthony C  et al. Molecular changes and vision loss in a mouse model of closed-globe blast trauma. Invest Ophthalmol Vis Sci 55:4853-62 (2014). Mouse . Read more (PubMed: 24994864) »

See 1 Publication for this product

Publishing research using ab34419? Please let us know so that we can cite the reference in this datasheet.

Customer reviews and Q&As

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4 reviews or Q&A

10
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unfortunately i have to inform you that the ChAT antibody (ab34419) didn't work even after following your suggestion to dilute the primary antibody in PBS instead of blocking solution. In your previous e-mail you recommended a few antibodies a...

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Thank you for contacting Abcam. I am sorry to hear that you are still not able to get any convincing staining using ab34419. I would be happy to get you a replacement, the ab70219, I am having a little difficulty finding your original order tho...

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Dear Dr Constable, thank you very much for your reply. Regarding your suggestions: I have used this secondary antibody before without problem and the fixation time i mentioned refers to the tissue i use for my experiments (mouse colon)....

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Thank you for updating me on the other protocol methods that you have tried. Please let me know how diluting the antibody in PBS rather than blocking buffer goes. I look forward to hearing from you and good luck with the staining toda

LOT NUMBER 811618 ORDER NUMBER -- NOT SPECIFIED -- DESCRIPTION OF THE PROBLEM No staining SAMPLE Mouse colon PRIMARY ANTIBODY Chicken polyclonal to Choline Acetyltransferase...

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Thank you for contacting Abcam about ab34419, Chicken polyclonal to Choline Acetyltransferase. I am sorry to hear that you are not able to get any staining using the antibody. I have looked over your methods that you provided and it seems that ...

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Dear Sir, Madam,   I am "an old customer" and I would like to enquire: I plan to study the protein expression of choline acetyltransferase in rat pancreas, in formalin fixed, paraffin-embedded tissue. Is there any of the various antibodies availa...

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Thank you very much for your interest in our products. The following antibodies are tested and guaranteed for IHC-P in rat. Unfortunately, we do not have blocking peptide for these antibodies in our catalogue. ab68779 Click here (or use...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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