Anti-Choline Acetyltransferase antibody (ab6168)

Overview

  • Product name
    Anti-Choline Acetyltransferase antibody
    See all Choline Acetyltransferase primary antibodies
  • Description
    Rabbit polyclonal to Choline Acetyltransferase
  • Specificity
    Extensive immunohistochemistry indicates localisation to cholinergic nervous system only. Will stain cholinergic neurons in rat central and peripheral nervous systems.
  • Tested applications
    Suitable for: ICC/IF, ELISA, IHC-P, IHC-Frmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Guinea pig
    Predicted to work with: Chicken, Human, Pig, Non human primatesDoes not react with: Sheep
  • Immunogen

    Synthetic peptide:

    H-GLFSSYRLPGHTQDTLVAQKSS

    conjugated to KLH by a Glutaraldehyde linker, corresponding to amino acids 168-189 of Choline Acetyltransferase.

  • Positive control
    • IHC: Rat forebrain tissue.
  • General notes
    This antibody appears to have identical characteristics to those described by Benecke et al (1993). The antigen is the same. It is an excellent reagent for immunohistochemistry for both peripheral and central nervous system, staining both cell bodies and nerve terminals.

Properties

Applications

Our Abpromise guarantee covers the use of ab6168 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent concentration. See Abreview.
ELISA 1/1000.
IHC-P Use at an assay dependent concentration. PubMed: 19422789
IHC-Fr 1/2000. Fix using formaldehyde. Perfusion fix the tissue with Zamboni fixative and post-fix (1-2hrs according to Masliukov PM & Timmermans) in 4% formaldehyde (4% formaldehyde with or without 15% picric acid is a very good fixative, it should also work with Bouin fixative too).Cryoprotect the tissue by direct immersion in 30% sucrose (at +4C). Use on free floating sections for best results.
This antibody does not work with glutaraldehyde fixation.

Target

Images

  • Immunohistochemistry analysis of free-floating rat forebrain tissue labelling Choline Acetyltransferase with ab6168 at a dilution of 1/100.

  • ab6168 staining rat stomach tissue sections by immunohistochemistry (Frozen sections). Tissue underwent fixation in paraformaldehyde. The primary antibody was diluted 1/200 and incubated with sample for 24 hours. A Texas Red® conjugated goat polyclonal (IgG whole molecule) to rabbit IgG, diluted 1/400 was used  as secondary. 

    See Abreview

References

This product has been referenced in:
  • Genabai NK  et al. Genetic inhibition of JNK3 ameliorates spinal muscular atrophy. Hum Mol Genet 24:6986-7004 (2015). Read more (PubMed: 26423457) »
  • Tu JL  et al. APOE 4 polymorphism results in early cognitive deficits in an EAE model. Biochem Biophys Res Commun 384:466-70 (2009). WB, IHC-P ; Mouse . Read more (PubMed: 19422789) »

See all 4 Publications for this product

Customer reviews and Q&As

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (human Mueller cells (MIO-M1))
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: citrate pH 6
Permeabilization
No
Specification
human Mueller cells (MIO-M1)
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
Fixative
10% NBF
Username

Abcam user community

Verified customer

Submitted Feb 17 2016

Application
Western blot
Sample
Rhesus monkey Tissue lysate - whole (macaque retina)
Gel Running Conditions
Reduced Denaturing (4-15%)
Loading amount
100 µg
Specification
macaque retina
Blocking step
Milk as blocking agent for 1 hour(s) and 30 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Monica Sauter

Verified customer

Submitted Jan 11 2016

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Cynomolgus Monkey Tissue sections (macaque retina)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: citrate pH 6
Permeabilization
No
Specification
macaque retina
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
Fixative
Paraformaldehyde
Username

Abcam user community

Verified customer

Submitted Dec 29 2015

Application
Immunohistochemistry (Frozen sections)
Blocking step
Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 4% · Temperature: 21°C
Sample
Mouse Tissue sections (Brain)
Specification
Brain
Permeabilization
Yes - 0.25 % Triton-x ind 0.1M PB
Fixative
Paraformaldehyde
Username

Abcam user community

Verified customer

Submitted Sep 10 2013

Thank you for your reply.

We have not validated ab6168 for use on perfused-fixed frozen sections (IHC-FoFr), and it would not be guaranteed for use in that application. The alternative antibody ab18736 is known to work well on perfused-fixed ...

Read More

Thank you for your email. I am sorry that this antibody is giving a weak signal. Could you please clarify a few points of your protocol?

1) What type of samples were tested? Were these rat brain sections or some other type of tissue? How long...

Read More

Thank you for contacting Abcam. I am sorry that you are having problems with ab6168 in IHC-P. I found the following reference that used the antibody successfully in IHC-P on mouse tissue: Tu JL et al. APOE 4 polymorphism results in early cognitive defi...

Read More

Thank you very much for your interest in our products. The following antibodies are tested and guaranteed for IHC-P in rat. Unfortunately, we do not have blocking peptide for these antibodies in our catalogue. ab68779 Click here (or use...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Frozen sections)
Sample
Rat Tissue sections (Stomach)
Specification
Stomach
Fixative
Paraformaldehyde
Permeabilization
No
Username

Prof. Marcin Arciszewski

Verified customer

Submitted Oct 27 2008

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Rat Cell (Brain sections)
Specification
Brain sections
Fixative
Paraformaldehyde
Permeabilization
No
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
Username

Dr. Li-Hsien Lin

Verified customer

Submitted Oct 10 2008

1-10 of 22 Abreviews or Q&A

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up