Overview

  • Product nameAnti-Choline kinase alpha antibody
    See all Choline kinase alpha primary antibodies
  • Description
    Rabbit polyclonal to Choline kinase alpha
  • Tested applicationsSuitable for: WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
    Predicted to work with: Zebrafish
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 400 to the C-terminus of Human Choline kinase alpha.

    (Peptide available as ab98290.)

  • Positive control
    • This antibody gave a positive signal in the following lysates: Human Breast Tissue; Mouse Lung Tissue; Mouse Brain Tissue; Rat Brain Tissue; MCF7 Whole Cell; HepG2 Whole Cell; HeLa Whole Cell; Jurkat Whole Cell; A549, Human Glioblastoma Whole Cell.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage bufferPreservative: 0.02% Sodium Azide
    Constituents: 1% BSA, PBS, pH 7.4
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Associated products

Applications

Our Abpromise guarantee covers the use of ab88053 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 52 kDa (predicted molecular weight: 52 kDa).
ICC/IF Use a concentration of 5 µg/ml.

Target

Anti-Choline kinase alpha antibody images

  • All lanes : Anti-Choline kinase alpha antibody (ab88053) at 1 µg/ml

    Lane 1 : MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate
    Lane 2 : Human breast tissue lysate - total protein (ab30090)
    Lane 3 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
    Lane 4 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 5 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
    Lane 6 : A549 (Human lung adenocarcinoma epithelial cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 52 kDa
    Observed band size : 52 kDa
    Additional bands at : 58 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 2 minutes
  • ICC/IF image of ab88053 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab88053, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899 Dylight 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
  • All lanes : Anti-Choline kinase alpha antibody (ab88053) at 1 µg/ml

    Lane 1 : Brain (Mouse) Tissue Lysate
    Lane 2 : Mouse lung normal tissue lysate - total protein (ab29297)
    Lane 3 : Brain (Rat) Tissue Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 52 kDa
    Observed band size : 52 kDa
    Additional bands at : 72 kDa,78 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 1 minute
  • Immunocytochemistry/ Immunofluorescence analysis of human glioblastoma cells labeling Choline kinase alpha with ab88053 at 1/250 dilution. Cells were fixed in paraformaldehyde and permeabilized with TritonX-100. A goat anti-rabbit Alexa Fluor594 was used as the secondary antibody at 1/1000 dilution.

    See Abreview

  • All lanes : Anti-Choline kinase alpha antibody (ab88053) at 1/1000 dilution

    Lane 1 : Choline kinase alpha knockdown Human Glioblastoma whole cell lysate
    Lane 2 : Human Glioblastoma (Control) whole cell lysate

    Lysates/proteins at 50 µg per lane.

    Secondary
    goat-anti-rabbit IRDye800CW at 1/10000 dilution

    Performed under reducing conditions.

    Predicted band size : 52 kDa


    Exposure time : 1 minute

    This image is courtesy of an anonymous Abreview

    See Abreview

References for Anti-Choline kinase alpha antibody (ab88053)

This product has been referenced in:
  • Zhang L  et al. CHKA mediates the poor prognosis of lung adenocarcinoma and acts as a prognostic indicator. Oncol Lett 12:1849-1853 (2016). WB ; Human . Read more (PubMed: 27588131) »
  • Koch K  et al. Reciprocal regulation of the cholinic phenotype and epithelial-mesenchymal transition in glioblastoma cells. Oncotarget N/A:N/A (2016). WB, ICC/IF ; Human . Read more (PubMed: 27705917) »

See all 3 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (Glioblastoma)
Permeabilization Yes - TritonX-100
Specification Glioblastoma
Blocking step Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 22°C
Fixative Paraformaldehyde
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Abcam user community

Verified customer

Submitted Oct 21 2016

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (Glioblastoma)
Gel Running Conditions Reduced Denaturing (4-12 % gradient gel)
Loading amount 50 µg
Specification Glioblastoma
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Username

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Verified customer

Submitted Oct 21 2016

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"