Anti-Choline kinase alpha antibody - Aminoterminal end (ab38290)

Overview

  • Product nameAnti-Choline kinase alpha antibody - Aminoterminal end
    See all Choline kinase alpha primary antibodies
  • Description
    Rabbit polyclonal to Choline kinase alpha - Aminoterminal end
  • Tested applicationsSuitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected within aa 50~150 of human Choline kinase alpha.

  • Positive control
    • Mouse heart tissue lysate.
  • General notes


    This antibody recognises the N-terminal region of CHK.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage bufferPreservative: 0.09% Sodium Azide
    Constituents: PBS
  • Concentration information loading...
  • PurityProtein G purified
  • Primary antibody notesThis antibody recognises the N-terminal region of CHK.
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab38290 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/100 - 1/500. Detects a band of approximately 48 kDa (predicted molecular weight: 52 kDa).
ELISA 1/1000.

Target

  • RelevanceThe dominant pathway for biosynthesis of phosphatidylcholine occurs through the CDP-choline pathway. Choline kinase alpha (CHK) is the first enzyme in the pathway and may thereby play an upstream regulatory role in lipid transport and metabolism. CHK additionally catalyzes phosphorylation of ethanolamine.
  • Cellular localizationCytoplasmic
  • Database links
  • Alternative names
    • CHETK alpha antibody
    • CHETK-alpha antibody
    • CHK antibody
    • CHKA antibody
    • CK antibody
    • CKI antibody
    • EK antibody
    • Ethanolamine kinase antibody
    see all

Anti-Choline kinase alpha antibody - Aminoterminal end images

References for Anti-Choline kinase alpha antibody - Aminoterminal end (ab38290)

This product has been referenced in:
  • Huang C  et al. Activation of choline kinase by extracellular Ca2+ is Ca(2+)-sensing receptor, Galpha12 and Rho-dependent in breast cancer cells. Cell Signal 21:1894-900 (2009). WB ; Human . Read more (PubMed: 19716891) »
  • Baykal AT  et al. Aberrant regulation of choline metabolism by mitochondrial electron transport system inhibition in neuroblastoma cells. Metabolomics 4:347-356 (2008). WB ; Human . Read more (PubMed: 19774105) »

See all 2 Publications for this product

Product Wall

Application Western blot
Sample Human Cell lysate - whole cell (breast cancer cell line)
Loading amount 30 µg
Specification breast cancer cell line
Gel Running Conditions Reduced Denaturing (8-16%)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Dorit Grunberger

Verified customer

Submitted Aug 05 2008

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"