The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/100 - 1/500. Detects a band of approximately 48 kDa (predicted molecular weight: 52 kDa).
The dominant pathway for biosynthesis of phosphatidylcholine occurs through the CDP-choline pathway. Choline kinase alpha (CHK) is the first enzyme in the pathway and may thereby play an upstream regulatory role in lipid transport and metabolism. CHK additionally catalyzes phosphorylation of ethanolamine.
Arlauckas SP et al. Near infrared fluorescent imaging of choline kinase alpha expression and inhibition in breast tumors. Oncotarget8:16518-16530 (2017).
Read more (PubMed: 28157707) »
Huang C et al. Activation of choline kinase by extracellular Ca2+ is Ca(2+)-sensing receptor, Galpha12 and Rho-dependent in breast cancer cells. Cell Signal21:1894-900 (2009).
Read more (PubMed: 19716891) »