The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/100 - 1/500. Detects a band of approximately 48 kDa (predicted molecular weight: 52 kDa).
RelevanceThe dominant pathway for biosynthesis of phosphatidylcholine occurs through the CDP-choline pathway. Choline kinase alpha (CHK) is the first enzyme in the pathway and may thereby play an upstream regulatory role in lipid transport and metabolism. CHK additionally catalyzes phosphorylation of ethanolamine.
References for Anti-Choline kinase alpha antibody - Aminoterminal end (ab38290)
This product has been referenced in:
Huang C et al. Activation of choline kinase by extracellular Ca2+ is Ca(2+)-sensing receptor, Galpha12 and Rho-dependent in breast cancer cells. Cell Signal21:1894-900 (2009).
Read more (PubMed: 19716891) »
Baykal AT et al. Aberrant regulation of choline metabolism by mitochondrial electron transport system inhibition in neuroblastoma cells. Metabolomics4:347-356 (2008).
Read more (PubMed: 19774105) »