Anti-Choline Oxidase antibody (Biotin) (ab181739)

Overview

  • Product name
    Anti-Choline Oxidase antibody (Biotin)
    See all Choline Oxidase primary antibodies
  • Description
    Goat polyclonal to Choline Oxidase (Biotin)
  • Host species
    Goat
  • Conjugation
    Biotin
  • Tested applications
    Suitable for: ELISA, WB, IPmore details
  • Species reactivity
    Reacts with: Other
  • Immunogen

    Full length native protein (purified) corresponding to Choline Oxidase aa 1-7. Alcaligenes species.
    Sequence:

    DNPNHSR


    Database link: P16101

  • Positive control
    • Alcaligenes spp. Choline Oxidase protein.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. Store In the Dark.
  • Storage buffer
    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 0.27% Potassium phosphate, 0.88% Sodium chloride, 1% BSA

    Immunoglobulin and protease free.
  • Concentration information loading...
  • Purity
    IgG fraction
  • Purification notes
    ab181739 is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipition arc against anti-Biotin anti-Goat Serum as well as purified and partially purified Alcaligenes spp. Choline Oxidase.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab181739 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/4000 - 1/20000.

ab181739 has been assayed against 1.0 µg of Alcaligenes spp. Choline Oxidase in a standard captured ELISA using Peroxidase Conjugated Streptavidin and ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature.

WB 1/500 - 1/5000. Predicted molecular weight: 0.8 kDa.
IP 1/100.

Target

  • Relevance
    Responsible for phosphatidylcholine synthesis via the CDP-choline pathway. Also exhibits ethanolamine kinase activity but at 14% efficiency compared with choline. ATP + choline = ADP + phosphocholine. Belongs to the choline/ethanolamine kinase family.
  • Cellular localization
    Cytoplasm.
  • Alternative names
    • L3130 antibody
    • Choline kinase antibody
    • CKI antibody
    • CKI1 antibody
    • L9606.8 antibody
    • YLR133W antibody
    see all

References

ab181739 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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