Anti-Chondroitin Sulfate antibody [CS-56] (ab11570)

Overview

  • Product nameAnti-Chondroitin Sulfate antibody [CS-56]
  • Description
    Mouse monoclonal [CS-56] to Chondroitin Sulfate
  • SpecificityThe antibody has been reported to be specific for the glycosaminoglycan (GAG) portion of native chondroitin sulfate proteoglycan (CSPG). The antibody reacts specifically with chondroitin sulfate types A and C but not with type B (dermatan sulfate), and may be used for localization of chondroitin sulfate in tissues and cultured fibroblasts.
  • Tested applicationsSuitable for: IHC-Fr, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Chicken, Cow, Human, Apteronotus leptorhynchus
  • Immunogen

    Ventral membranes of chicken gizzard fibroblasts.

  • Positive control
    • Bovine mammary gland epithelial (BMGE) cells.
  • General notes

    This product was changed from ascites to tissue culture supernatant on 25th October 2016. The following lot(s) is/are from ascites and is still in stock as of 25th October 2016 - GR288674, GR272407. Lot numbers higher than GR288674, GR272407 will be from tissue culture supernatant. Please note that the dilutions may need to be adjusted accordingly

    Storage in frost-free freezers is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use.

    Many cellular activities depend on the interaction of cells with the surrounding extracellular matrix (ECM). Most cells, in intact tissue and in culture, are attached to an ECM. Epithelial cells are associated with the basement membrane; fibroblastic cells are usually embedded in a pericellular mesh of fibrils, and tissue culture cells usually grow on a substrate which is covered by various ECM components. Studies have indicated that the matrix or its various isolated components provide not only adhesive surfaces for cells to grow on but also have effects on the rate of cell growth, mobility, morphogenesis and differentiation. Within the ECM several glycoproteins and proteoglycans have been identified. It has been proposed that the different constituents interact with each other in a rather complex fashion. The poor antigenicity of proteoglycans especially their glycoaminoglycan (GAG) moieties make it difficult to localize these molecules in tissue and cell culture. Monoclonal Anti-Chondroitin Sulfate can be used to study chondroitin sulfate proteoglycan (CSPG) distribution and its relationships to specific cell-substrate contacts.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferPreservative: 15mM Sodium Azide
    Constituents: Ascites fluid
  • PurityTissue culture supernatant
  • Primary antibody notesMany cellular activities depend on the interaction of cells with the surrounding extracellular matrix (ECM). Most cells, in intact tissue and in culture, are attached to an ECM. Epithelial cells are associated with the basement membrane; fibroblastic cells are usually embedded in a pericellular mesh of fibrils, and tissue culture cells usually grow on a substrate which is covered by various ECM components. Studies have indicated that the matrix or its various isolated components provide not only adhesive surfaces for cells to grow on but also have effects on the rate of cell growth, mobility, morphogenesis and differentiation. Within the ECM several glycoproteins and proteoglycans have been identified. It has been proposed that the different constituents interact with each other in a rather complex fashion. The poor antigenicity of proteoglycans especially their glycoaminoglycan (GAG) moieties make it difficult to localize these molecules in tissue and cell culture. Monoclonal Anti-Chondroitin Sulfate can be used to study chondroitin sulfate proteoglycan (CSPG) distribution and its relationships to specific cell-substrate contacts.
  • ClonalityMonoclonal
  • Clone numberCS-56
  • IsotypeIgM
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab11570 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr 1/200. PubMed: 16237170
The antibody has been reported to be specific for the glycosaminoglycan (GAG) portion of native chondroitin sulfate proteoglycan (CSPG). The antibody reacts specifically with chondroitin sulfate types A and C but not with type B (dermatan sulfate), and may be used for localization of chondroitin sulfate in tissues and cultured fibroblasts.
IHC-P Use at an assay dependent concentration.
ICC/IF Use at an assay dependent dilution.

Anti-Chondroitin Sulfate antibody [CS-56] images

  • ab11570 staining Chondroitin Sulfate in murine brain tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).
    For more detail please see abreview.

    See Abreview

  • ab11570 staining Chondroitin Sulfate in 16 µm thick transverse sections of Apteronotus leptorhynchus whole body tissue by Immunohistochemistry (Frozen sections).
    Tissue was fixed in 2% paraformaldehyde, blocked with 3% sheep serum, 1% BSA, 1% teleostean gelatine in TBS for 1 hour at 24°C and then incubated with ab11570 at a 1/50 dilution for 18 hours at 4°C. The secondary used was an Alexa-Fluor 546 conjugated goat anti-mouse polyclonal used at a 1/200 dilution.

    See Abreview

  • Coronal section of the developing cerebral cortex of an embryonic day 16 rat stained with Monoclonal Anti-Chondroitin Sulfate, ab11570, followed by fluorescein labeled Goat Anti-Mouse Ig. The chondroitin sulfate proteoglycan (CSPG) is most concentrated in the pia matter and the sub-plate (bright green layers). There is moderate staining in the intermediate zone, and there is very little CSPG in the cortical plate and ventricular zone. The cortex was stained with bis-benzimide that labels the cell nucleus and fluoresces blue under ultraviolet illumination
  • Immunoperoxidase staining of a frozen section of normal rabbit aorta stained using Monoclonal Anti-Chondroitin Sulfate ab11570.
  • Normal rabbit aorta, Lowicryl K4M thin section, stained with Monoclonal Anti-Chondroitin Sulfate, ab11570 and Goat Anti-Mouse IgM (µ-chain specific) 10 nm gold. Counterstain was uranyl acetate and Reynold's lead citrate. Magnification 44,600x. (el=Elastin, col=Collagen).

References for Anti-Chondroitin Sulfate antibody [CS-56] (ab11570)

This product has been referenced in:
  • Winkler T  et al. Wnt signaling activates Shh signaling in early postnatal intervertebral discs, and re-activates Shh signaling in old discs in the mouse. PLoS One 9:e98444 (2014). IHC-Fr ; Mouse . Read more (PubMed: 24892825) »
  • Dahia CL  et al. Shh signaling from the nucleus pulposus is required for the postnatal growth and differentiation of the mouse intervertebral disc. PLoS One 7:e35944 (2012). ICC/IF ; Mouse . Read more (PubMed: 22558278) »

See all 9 Publications for this product

Product Wall

Application Western blot
Sample Apteronotus leptorhynchus Tissue lysate - whole (Brain)
Loading amount 50 µg
Specification Brain
Gel Running Conditions Reduced Denaturing (4-15%)
Blocking step Milk as blocking agent for 14 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Username

Abcam user community

Verified customer

Submitted Nov 27 2012

Vielen Dank für Ihren Anruf und für Ihr Interesse an unseren Produkten.

Ich freue mich Ihnen bestätigen zu können, dass ab11570 (Anti-Chondroitin Sulfate antibody [CS-56]) für Glykosaminoglykan (GAG) ist, unabhän...

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Unfortunately we have not tested this product with digested chondroitin sulphate and so it is difficult to predict the outcome. GAGs alone are poorly antigenic and so the antibody may not interact with the D-glucuronic acid and N-acetyl-D-galactosamine...

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Thank you for contacting us yesterday in regards to the Anti-Chondroitin Sulfate antibody [CS-56] (ab11570).

I have had a look into which tissues may serve as positive controls with the antibody. As the Chondroitin sulfate is a major componen...

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Thank you for your enquiry.

The 1.7mg/ml concentration is the antibody concentration which was determined by


Radial immunodiffusion (RID) method. For your information, the ascites concentration with other proteins included is...

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Thank you for contacting us.
Please be advised that ab11570 is not a Chondroitin protein that will be degraded by chondroitinase ABC enzyme. It is an IgM antibody.
I hope this information is helpful to you. Please do not hesitate to contact u...

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Thank you for your reply.

I can confirm that Anti-Chondroitin Sulfate antibody [CS-56], (ab11570) has been raised against the chicken protein. I am sorry this antibody has been sources externally, and regrettably we are not able to obtain th...

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Thank you for contacting us. We need more information on the use of ab11570 and ab8610 in alphaLISA. Could you please answer the following questions? - Are you going to use these antibodies together or in combination with other antibodies? - ...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Frozen sections)
Sample Apteronotus leptorhynchus Tissue sections (Whole body)
Specification Whole body
Fixative Paraformaldehyde
Blocking step 3% sheep serum, 1% BSA, 1% teleostean gelatine in TBS as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 24°C
Username

Dr. Ruxandra Sirbulescu

Verified customer

Submitted Nov 14 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Mouse Tissue sections (Brain)
Specification Brain
Fixative microwave euthanasia
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Trilogy
Permeabilization Yes - tween20 (0.05%)
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 22°C
Username

Mr. Adrian Epstein

Verified customer

Submitted Aug 02 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"