Overview

  • Product name
  • Description
    Rabbit polyclonal to Chordin
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
    Predicted to work with: Sheep, Horse, Cow, Dog, Macaque monkey
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 450 - 550 of Human Chordin.

  • Positive control
    • This antibody gave a positive signal in the following tissue lysates: Human, Mouse and Rat brain; Mouse and Rat liver.

Properties

Applications

Our Abpromise guarantee covers the use of ab101316 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 110 kDa (predicted molecular weight: 102 kDa).

Target

  • Function
    Dorsalizing factor. Key developmental protein that dorsalizes early vertebrate embryonic tissues by binding to ventralizing TGF-beta family bone morphogenetic proteins (BMPs) and sequestering them in latent complexes.
  • Tissue specificity
    Expressed at the highest level in liver.
  • Sequence similarities
    Belongs to the chordin family.
    Contains 4 CHRD domains.
    Contains 4 VWFC domains.
  • Post-translational
    modifications
    Cleaved by tolloid proteases; cleavage participates to dorsoventral patterning during early development.
  • Cellular localization
    Secreted.
  • Information by UniProt
  • Database links
  • Alternative names
    • Chordin antibody
    • chrd antibody
    • CHRD_HUMAN antibody
    • MGC133038 antibody
    • Shortgastrulation protein antibody
    • SOG antibody
    see all

Anti-Chordin antibody images

  • All lanes : Anti-Chordin antibody (ab101316) at 1 µg/ml

    Lane 1 : Human brain tissue lysate - total protein (ab29466)
    Lane 2 : Liver (Mouse) Tissue Lysate
    Lane 3 : Liver (Rat) Tissue Lysate
    Lane 4 : Brain (Mouse) Tissue Lysate
    Lane 5 : Brain (Rat) Tissue Lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 102 kDa
    Observed band size : 110 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 46 kDa,80 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 12 minutes

References for Anti-Chordin antibody (ab101316)

ab101316 has not yet been referenced specifically in any publications.

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application
IHC - Wholemount
Sample
Chicken Cell (Whole Embryo)
Specification
Whole Embryo
Username

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Verified customer

Submitted May 08 2017

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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