This antibody gave a positive signal in the following tissue lysates: Human, Mouse and Rat brain; Mouse and Rat liver.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40 Preservative: 0.02% Sodium azide Constituent: PBS Note: Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 µg/ml. Detects a band of approximately 110 kDa (predicted molecular weight: 102 kDa).
FunctionDorsalizing factor. Key developmental protein that dorsalizes early vertebrate embryonic tissues by binding to ventralizing TGF-beta family bone morphogenetic proteins (BMPs) and sequestering them in latent complexes.
Tissue specificityExpressed at the highest level in liver.
Sequence similaritiesBelongs to the chordin family. Contains 4 CHRD domains. Contains 4 VWFC domains.
Post-translational modificationsCleaved by tolloid proteases; cleavage participates to dorsoventral patterning during early development.
All lanes : Anti-Chordin antibody (ab101316) at 1 µg/ml
Lane 1 : Human brain tissue lysate - total protein (ab29466) Lane 2 : Liver (Mouse) Tissue Lysate Lane 3 : Liver (Rat) Tissue Lysate Lane 4 : Brain (Mouse) Tissue Lysate Lane 5 : Brain (Rat) Tissue Lysate
Lysates/proteins at 20 µg per lane.
Secondary Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution Developed using the ECL technique