Chorionic Gonadotropin Human ELISA Kit (ab108636)

Overview

  • Product nameChorionic Gonadotropin Human ELISA KitSee all Chorionic Gonadotropin kits ...
  • Detection methodColorimetric
  • Tests
    1 x 96 well plate
  • Sample type
    Serum
  • Assay typeSandwich (quantitative)
  • Sensitivity
    = 2 mU/ml
  • Range
    2 mU/ml - 300 mU/ml
  • Assay durationMultiple steps standard assay
  • Species reactivity
    Reacts with: Human
  • Product overview

    Abcam’s Chorionic Gonadotropin in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of Chorionic Gonadotropin in Human serum.

    A 96-well plate has been precoated with anti-Chorionic Gonadotropin antibodies. Samples and standards and are added to the wells, where Chorionic Gonadotropin in the sample and standards binds to the precoated antibody. After incubation and washing, added Anti-Chorionic Gonadotropin HRP conjugate binds to the antibody-Chorionic Gonadotropin complex. After incubation, the wells are washed to remove unbound material and TMB substrate is then added which is catalyzed by HRP to produce blue coloration. The reaction is terminated by addition of Stop Solution which stops the color development and produces a color change from blue to yellow. The intensity of signal is directly proportional to the amount of Chorionic Gonadotropin in the sample and the intensity is measured at 450 nm.

     

  • Tested applicationsSandwich ELISAmore details
  • PlatformMicroplate

Properties

  • Storage instructionsStore at +4°C. Please refer to protocols.
  • Components 1 x 96 tests
    Anti-Chorionic Gonadotropin Coated Microplate (12 x 8 wells) 1 unit
    Anti-Chorionic Gonadotropin HRP Conjugate 1 x 18ml
    Chorionic Gonadotropin Standard 0 - 0 mU/mL 1 vial
    Chorionic Gonadotropin Standard 1 - 5 mU/mL 1 vial
    Chorionic Gonadotropin Standard 2 - 20 mU/mL 1 vial
    Chorionic Gonadotropin Standard 3 - 50 mU/mL 1 vial
    Chorionic Gonadotropin Standard 4 - 150 mU/mL 1 vial
    Chorionic Gonadotropin Standard 5 - 300 mU/mL 1 vial
    Stop Solution 1 x 11ml
    TMB Substrate Solution 1 x 11ml
    Zero Buffer 1 x 13ml
  • Research Areas

Applications

Our Abpromise guarantee covers the use of ab108636 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent dilution.

Chorionic Gonadotropin Human ELISA Kit images

  • Representative standard curve using ab108636

Protocols

References for Chorionic Gonadotropin Human ELISA Kit (ab108636)

ab108636 has not yet been referenced specifically in any publications.

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Unfortunately neither the Chorionic Gonadotropin Human ELISA Kit ab108636 and ab108637 has been tested with any other samples other than from human origin. As we have ...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"