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Synthetic peptide conjugated to KLH derived from within residues 900 to the C-terminus of Schizosaccharomyces pombe Chp1.
(Peptide available as ab19117.)
Our Abpromise guarantee covers the use of ab18191 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ChIP||Use 2.5µg for 107 cells.|
|WB||Use a concentration of 1 µg/ml. Detects a band of approximately 109 kDa (predicted molecular weight: 109 kDa).|
X-ChIP performed with ab18191 on Schizosaccharomyces pombe Cell lysate (whole cell). 5µl antibody were used per 500µl of chromatin/cell lysate according to standard S. pombe ChIP protocols. Protein-A Sepharose was used to recover the ChIPs. In the positive control the researcher specifically induced silencing of the ura4+ gene. For the negative control the researcher performed multiplex PCR with primers amplifying ura4+ and an un-silenced gene. In addition, act1+ was amplified in a separate PCR reaction. Chp1 only binds to ura4+ (and only if silencing is induced).
This image is courtesy of an Abreview submitted by Marc B? on 9 February 2006
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"