Overview

  • Product name
  • Description
    Rabbit polyclonal to CHRAC1
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Rat, Cow, Macaque monkey, Bat
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human CHRAC1.

  • Positive control
    • This antibody gave a positive signal in the following human whole cell lysates: HeLa; HepG2; HEK293.

Properties

Applications

Our Abpromise guarantee covers the use of ab105452 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 14 kDa (predicted molecular weight: 14 kDa).

Target

  • Function
    Forms a complex with DNA polymerase epsilon subunit POLE3 and binds naked DNA, which is then incorporated into chromatin, aided by the nucleosome remodeling activity of ISWI/SNF2H and ACF1.
  • Tissue specificity
    Expressed in all tissues tested, including, heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • CHARC1 antibody
    • CHARC15 antibody
    • CHRAC-1 antibody
    • CHRAC-15 antibody
    • Chrac1 antibody
    • CHRAC15 antibody
    • CHRC1_HUMAN antibody
    • Chromatin accessibility complex 1 antibody
    • Chromatin accessibility complex 15 kDa protein antibody
    • Chromatin accessibility complex protein 1 antibody
    • DNA polymerase epsilon subunit p15 antibody
    • histone-fold protein CHRAC15 antibody
    • HuCHRAC15 antibody
    • YCL1 antibody
    see all

Images

  • All lanes : Anti-CHRAC1 antibody (ab105452) at 1 µg/ml

    Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 2 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
    Lane 3 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 14 kDa
    Observed band size : 14 kDa
    Additional bands at : 80 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 4 minutes

References

ab105452 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab105452.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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