Overview

  • Product nameAnti-CHRAC1 antibody
    See all CHRAC1 primary antibodies
  • Description
    Rabbit polyclonal to CHRAC1
  • Tested applicationsSuitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Horse, Guinea pig, Cow, Dog
  • Immunogen

    Synthetic peptide from within the internal sequence amino acids 36-85 (SINQEALVLT AKATELFVQC LATYSYRHGS GKEKKVLTYS DLANTAQQSE) of Human CHRAC1 (accession number: NP_059140)

  • Positive control
    • Fetal heart lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab83079 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 15 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA Use at an assay dependent concentration.

Titre using peptide based assay: 1:312500.

Target

  • FunctionForms a complex with DNA polymerase epsilon subunit POLE3 and binds naked DNA, which is then incorporated into chromatin, aided by the nucleosome remodeling activity of ISWI/SNF2H and ACF1.
  • Tissue specificityExpressed in all tissues tested, including, heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas.
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • CHARC1 antibody
    • CHARC15 antibody
    • CHRAC-1 antibody
    • CHRAC-15 antibody
    • Chrac1 antibody
    • CHRAC15 antibody
    • CHRC1_HUMAN antibody
    • Chromatin accessibility complex 1 antibody
    • Chromatin accessibility complex 15 kDa protein antibody
    • Chromatin accessibility complex protein 1 antibody
    • DNA polymerase epsilon subunit p15 antibody
    • histone-fold protein CHRAC15 antibody
    • HuCHRAC15 antibody
    • YCL1 antibody
    see all

Anti-CHRAC1 antibody images

  • Anti-CHRAC1 antibody (ab83079) at 1 µg/ml + Human fetal heart lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 15 kDa
    Observed band size : 20 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 40 kDa. We are unsure as to the identity of these extra bands.

References for Anti-CHRAC1 antibody (ab83079)

ab83079 has not yet been referenced specifically in any publications.

Product Wall

Application Western blot
Loading amount 40 µg
Gel Running Conditions Non-reduced Denaturing
Sample Mouse Cell lysate - whole cell (Spleen)
Specification Spleen
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 26°C
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Submitted Apr 10 2015

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"