The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ChIP: Use at an assay dependent dilution.
ICC/IF: 1/100 - 1/500.
IHC-P: Use at an assay dependent dilution.
WB: Use at an assay dependent dilution. Predicted molecular weight: 95 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Transcriptional repressor. Binds to the canonical and non-canonical E box sequences 5'-CACGTG-3'.
Expressed in liver, heart, kidney, cerebellum and intestinal tissues.
Involvement in disease
Note=WBSCR14 is located in the Williams-Beuren syndrome (WBS) critical region. WBS results from a hemizygous deletion of several genes on chromosome 7q11.23, thought to arise as a consequence of unequal crossing over between highly homologous low-copy repeat sequences flanking the deleted region. Haploinsufficiency of WBSCR14 may be the cause of certain cardiovascular and musculo-skeletal abnormalities observed in the disease.
Zhang Y et al. Berberine reverses abnormal expression of L-type pyruvate kinase by DNA demethylation and histone acetylation in the livers of the non-alcoholic fatty disease rat. Int J Clin Exp Med8:7535-43 (2015).
Read more (PubMed: 26221297) »
Erion DM et al. The role of the carbohydrate response element-binding protein in male fructose-fed rats. Endocrinology154:36-44 (2013).
Read more (PubMed: 23161873) »