Overview

  • Product nameAnti-CHRFAM7A antibody
    See all CHRFAM7A primary antibodies
  • Description
    Rabbit polyclonal to CHRFAM7A
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Zebrafish
  • Immunogen

    Synthetic peptide derived from residues 11 - 60 of human CHRFAM7A.

  • Positive control
    • Jurkat cell lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab28885 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.5 µg/ml. Detects a band of approximately 40 kDa (predicted molecular weight: 45 - 56 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

  • RelevanceThe nicotinic acetylcholine receptors (nAChRs) are members of a superfamily of ligand-gated ion channels that mediate fast signal transmission at synapses. CHRFAM7A is encoded by a hybrid gene consisting of a duplication of exons 5 through 10 of the CHRNA7 gene fused 3-prime to a copy of the FAM7A gene (exons A through E). Alternative splicing has been observed, and two variants exist, for this hybrid gene. The N-terminally truncated products predicted by the largest open reading frames for each variant would lack the majority of the neurotransmitter-gated ion-channel ligand binding domain but retain the transmembrane region that forms the ion channel.
  • Cellular localizationMembrane; Multi-pass membrane protein.
  • Database links
  • Alternative names
    • Alpha 7 neuronal nicotinic acetylcholine receptor FAM7A hybrid antibody
    • CHRNA7 (cholinergic receptor nicotinic alpha 7 exons 5 10) and FAM7A (family with sequence similarity 7A exons A E) fusion antibody
    • CHRNA7 antibody
    • CHRNA7 DR1 antibody
    • CHRNA7 FAM7A fusion antibody
    • CHRNA7 FAM7A fusion protein antibody
    • D 10 antibody
    • D10 antibody
    • MGC120482 antibody
    • MGC120483 antibody
    see all

Anti-CHRFAM7A antibody images

  • Anti-CHRFAM7A antibody (ab28885) at 0.5 µg/ml + Jurkat cell lysate

    Secondary
    HRP conjugated anti-Rabbit IgG (suggested dilution 1:50000 - 100000)
    Developed using the ECL technique

    Predicted band size : 45 - 56 kDa
    Observed band size : 40 kDa (why is the actual band size different from the predicted?)

References for Anti-CHRFAM7A antibody (ab28885)

ab28885 has not yet been referenced specifically in any publications.

Product Wall

This is correct, the Uniprot reference for the human CHRFAM7A is Q494W8. Please note that the exact immunogen is proprietary, the information given on the datasheet (aa 11-60) is the region where the exact sequence is derived from. According to the...

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Upon Blasting the peptide immunogen used to generate this antibody we found that it is 100% homologous to the human CHRNA7 protein (NCBI gene ID: 1139) and 100% homologous to the fusion protein CHRFAM7A (NCBI gene ID: 89832).  The next highest homolo...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"