Anti-CHRNA6 antibody [5B6G8] (ab233721)
Key features and details
- Mouse monoclonal [5B6G8] to CHRNA6
- Suitable for: ICC/IF, Flow Cyt, WB, IHC-P
- Reacts with: Human
- Isotype: IgG1
Overview
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Product name
Anti-CHRNA6 antibody [5B6G8]
See all CHRNA6 primary antibodies -
Description
Mouse monoclonal [5B6G8] to CHRNA6 -
Host species
Mouse -
Tested applications
Suitable for: ICC/IF, Flow Cyt, WB, IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment corresponding to Human CHRNA6 aa 26-239. (Expressed in E.coli).
Database link: Q15825 -
Positive control
- WB: Recombinant human CHRNA6 (aa 26-239) protein; CHRNA6 (aa 26-239)-hIgGFc transfected HEK-293 cell lysate. IHC-P: Human bladder cancer tissue. ICC/IF: HeLa cells. Flow Cyt: SH-SY5Y and SK-N-SH cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.05% Sodium azide
Constituent: PBS -
Concentration information loading...
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Purity
Protein G purified -
Purification notes
Purified from tissue culture supernatant. -
Clonality
Monoclonal -
Clone number
5B6G8 -
Isotype
IgG1 -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab233721 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ICC/IF |
1/100 - 1/500.
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Flow Cyt |
1/200 - 1/400.
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WB |
1/500 - 1/2000. Predicted molecular weight: 57 kDa.
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IHC-P |
1/200 - 1/1000.
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Notes |
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ICC/IF
1/100 - 1/500. |
Flow Cyt
1/200 - 1/400. |
WB
1/500 - 1/2000. Predicted molecular weight: 57 kDa. |
IHC-P
1/200 - 1/1000. |
Target
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Relevance
Nicotinic acetylcholine receptors (nAChRs, AChRs) are protein complexes made up of protein subunits that coassemble to form an ion channel. This ion channel is gated through the binding of the neurotransmitter acetylcholine (ACh) to its ligand-binding site on the AChR. After binding acetylcholine, the AChR responds by an extensive change in conformation that affects all subunits and leads to opening of an ion-conducting channel across the plasma membrane. CHRNA6 (cholinergic nicotinic receptor alpha polypeptide 6) is a neuronal acetylcholine receptor protein. -
Cellular localization
Cell junction, synapse, postsynaptic cell membrane; Multi-pass membrane protein. Cell membrane; Multi-pass membrane protein. -
Database links
- Entrez Gene: 8973 Human
- Omim: 606888 Human
- SwissProt: Q15825 Human
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Alternative names
- AChR-alpha6 antibody
- Acra6 antibody
- Cholinergic receptor nicotinic alpha 6 antibody
see all
Images
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Anti-CHRNA6 antibody [5B6G8] (ab233721) + Recombinant human CHRNA6 (aa 26-239) protein
Predicted band size: 57 kDaExpected MW is 51.2 kDa.
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All lanes : Anti-CHRNA6 antibody [5B6G8] (ab233721) at 1/500 dilution
Lane 1 : HEK-293 (human epithelial cell line from embryonic kidney) cell lysate
Lane 2 : CHRNA6 (aa 26-239)-hIgGFc transfected HEK HEK-293 cell lysate
Predicted band size: 57 kDa -
HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for CHRNA6 (green) using ab233721 at 1/100 dilution in ICC/IF.
Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
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Flow cytometric analysis of SH-SY5Y (human neuroblastoma cell line from bone marrow) cell line labeling CHRNA6 with ab233721 at 1/200 dilution (green) compared with a negative control (red).
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Flow cytometric analysis of SK-N-SH (human neuroblastoma cell line) cell line labeling CHRNA6 with ab233721 at 1/200 dilution (green) compared with a negative control (red).
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Paraffin-embedded human bladder cancer tissue stained for CHRNA6 using ab233721 at 1/200 dilution in immunohistochemical analysis. DAB staining.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
References (0)
ab233721 has not yet been referenced specifically in any publications.