Anti-Chromodomain helicase DNA binding protein 5 antibody (ab66516)

Overview

  • Product nameAnti-Chromodomain helicase DNA binding protein 5 antibody
    See all Chromodomain helicase DNA binding protein 5 primary antibodies
  • Description
    Rabbit polyclonal to Chromodomain helicase DNA binding protein 5
  • Tested applicationsSuitable for: IP, WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Horse, Cow, Dog, Chimpanzee
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 1550 - 1650 of Human Chromodomain helicase DNA binding protein 5.

    (Peptide available as ab69729.)

  • Positive control
    • This antibody gave a positive signal in human liver tissue lysate and in the following cell lysates: TE 671 Whole Cell; HeLa Whole Cell; HeLa Whole Cell - Hydroxyurea Treated (48hr, 2uM); HeLa Whole Cell - Bleomycin Treated (20U/ml); HeLa nuclear; Jurkat Whole Cell; Jurkat nuclear; HepG2 Whole Cell; JEG3 Whole Cell.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage bufferPreservative: 0.02% Sodium Azide
    Constituents: 1% BSA, PBS, pH 7.4
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab66516 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use at an assay dependent concentration.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 250 kDa (predicted molecular weight: 223 kDa).

Target

  • FunctionMay play a role in the development of the nervous system and the pathogenesis of neural tumors.
  • Tissue specificityPreferentially expressed in total brain, fetal brain, and cerebellum. It is also moderately expressed in the adrenal gland.
  • Sequence similaritiesBelongs to the SNF2/RAD54 helicase family.
    Contains 2 chromo domains.
    Contains 1 helicase ATP-binding domain.
    Contains 1 helicase C-terminal domain.
    Contains 2 PHD-type zinc fingers.
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • ATP-dependent helicase CHD5 antibody
    • CHD 5 antibody
    • CHD-5 antibody
    • CHD5 antibody
    • CHD5_HUMAN antibody
    • Chromodomain-helicase-DNA-binding protein 5 antibody
    • DKFZp434N231 antibody
    • KIAA0444 antibody
    • RP1-233K16.2 antibody
    see all

Anti-Chromodomain helicase DNA binding protein 5 antibody images

  • All lanes : Anti-Chromodomain helicase DNA binding protein 5 antibody (ab66516) at 1 µg/ml

    Lane 1 : TE 671 (Human Rhabdomyosarcoma) Whole Cell Lysate
    Lane 2 : Hela Whole Cell Lysate - Hydroxyurea Treated (48hr, 2uM)
    Lane 3 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
    Lane 4 : Hela Whole Cell Lysate - Bleomycin Treated (20U/ml)
    Lane 5 : Human liver tissue lysate - total protein (ab29889)
    Lane 6 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
    Lane 7 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 8 : JEG-3 (Human placental choriocarcinoma cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Performed under reducing conditions.

    Predicted band size : 223 kDa
    Observed band size : 250 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 150 kDa,220 kDa,60 kDa. We are unsure as to the identity of these extra bands.
  • All lanes : Anti-Chromodomain helicase DNA binding protein 5 antibody (ab66516) at 1 µg/ml

    Lane 1 : HeLa (Human epithelial carcinoma cell line) Nuclear Lysate
    Lane 2 : Jurkat nuclear extract lysate (ab14844)

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP), pre-adsorbed at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 223 kDa
    Observed band size : 240 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 58 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 2 minutes
  • Chromodomain helicase DNA binding protein 5 was immunoprecipitated using 0.5mg Hela whole cell extract, 5µg of Rabbit polyclonal to Chromodomain helicase DNA binding protein 5 and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).
    The antibody was incubated under agitation with Protein G beads for 10min, Hela whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.
    Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70oC; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab66516.
    Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697).
    Band: 240kDa: Chromodomain helicase DNA binding protein 5; non specific - 58kDa: We are unsure as to the identity of this extra band.

References for Anti-Chromodomain helicase DNA binding protein 5 antibody (ab66516)

This product has been referenced in:
  • Cai C  et al. MicroRNA-211 expression promotes colorectal cancer cell growth in vitro and in vivo by targeting tumor suppressor CHD5. PLoS One 7:e29750 (2012). WB ; Human . Read more (PubMed: 22235338) »

See 1 Publication for this product

Product Wall

Thank you very much for your call yesterday and for your interest in ab66516.

I've looked through the literature and discussed with the lab scientists, andthere is no specific reason why the antibody was tested on hydroxyurea and bleomycin-tr...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"