Overview

  • Product name
    Anti-Chromogranin A antibody
    See all Chromogranin A primary antibodies
  • Description
    Rabbit polyclonal to Chromogranin A
  • Tested applications
    Suitable for: IHC-Fr, WB, ICC/IF, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Human, Monkey
  • Immunogen

    Recombinant fragment from the C-terminal (Human)

  • Positive control
    • Pancreas

Properties

Applications

Our Abpromise guarantee covers the use of ab15160 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr Use at an assay dependent concentration. PubMed: 21383188
WB 1/100.
ICC/IF 1/100.
IHC-P 1/400.

Target

  • Function
    Pancreastatin strongly inhibits glucose induced insulin release from the pancreas.
  • Sequence similarities
    Belongs to the chromogranin/secretogranin protein family.
  • Post-translational
    modifications
    Sulfated on tyrosine residues and/or contains sulfated glycans.
    O-glycosylated with core 1 or possibly core 8 glycans.
  • Cellular localization
    Secreted. Neuroendocrine and endocrine secretory granules.
  • Information by UniProt
  • Database links
  • Form
    According to the Swiss-Prot database, this protein has many different forms which correspond to various molecular weights; thus the possible variation from the predicted molecular weight of 51kDa.
  • Alternative names
    • beta Granin antibody
    • betagranin (N-terminal fragment of chromogranin A) antibody
    • catestatin antibody
    • CgA antibody
    • CHG A antibody
    • Chga antibody
    • chromofungin antibody
    • Chromogranin A antibody
    • Chromogranin A parathyroid secretory protein 1 antibody
    • Chromogranin A precursor antibody
    • ChromograninA antibody
    • CMGA_HUMAN antibody
    • ER-37 antibody
    • Pancreastatin antibody
    • Parastatin antibody
    • Parathyroid secretory protein 1 antibody
    • Pituitary secretory protein I antibody
    • Secretory protein I antibody
    • SP I antibody
    • SP-I antibody
    • SP1 antibody
    • SPI antibody
    • Vasostatin antibody
    • Vasostatin I antibody
    • Vasostatin II antibody
    see all

Anti-Chromogranin A antibody images

  • ab15160 at 1/250 staining human pancreas tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step was performed before incubation with the antibody for 30 minutes at 25°C. An HRP conjugated rabbit polyclonal was used as the secondary.

    See Abreview

  • ab15160 staining Chromogranin A in mouse gastric gland cells by Immunocytochemistry/ Immunofluorescence. Cells were fixed with paraformaldehyde, permeabilized with 0.2% Triton ×100 and blocking with 10% serum for 45min at 200C was performed. Samples were incubated with primary antibody (1/100) for 18 hours at 4°C. A Texas Red ®-conjugated goat polyclonal to rabbit IgG was used at dilution 1/200 as secondary antibody.

    See Abreview

  • ab15160 (1/100) staining Chromogranin A in paraffin-embbeded mouse pancreas tissue sections. Tissue underwent fixation in formaldehyde, heat-mediated antigen retrieval in citrate buffer pH 6.0 and blocking (10 minutes, 1.5% serum). An HRP-conjugated goat polyclonal was used as the secondary. For further experimental details please refer to abreview.

    See Abreview

  • ab15160 staining Chromogranin A in mouse colon tissue section by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Tissue underwent formalin fixation before heat mediated antigen retrieval with 10mM citric acid buffer and then the sample was blocked for 45 minutes at 20°C. The primary antibody (1/100) was incubated with sample in 10% serum for 18 hours at 4°C. A Biotin conjugated goat polyclonal to rabbit IgG was used at dilution 1/200 as secondary antibody.

    See Abreview


  • Developed using the ECL technique

    Performed under reducing conditions.

    Exposure time : 30 secondsChromogranin A is easily fragmented and both bands observed in the Western Blot above are believed to correspond to Chromogranin A.

References for Anti-Chromogranin A antibody (ab15160)

This product has been referenced in:
  • Miyoshi H  et al. Prostaglandin E2 promotes intestinal repair through an adaptive cellular response of the epithelium. EMBO J 36:5-24 (2017). Read more (PubMed: 27797821) »
  • Yuan H  et al. HPV positive neuroendocrine cervical cancer cells are dependent on Myc but not E6/E7 viral oncogenes. Sci Rep 7:45617 (2017). IHC-P ; Human . Read more (PubMed: 28378747) »

See all 33 Publications for this product

Product Wall

Application
IHC - Wholemount
Sample
Mouse Tissue (intestine organoids)
Specification
intestine organoids
Username

Abcam user community

Verified customer

Submitted Mar 29 2017

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (colon)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Sodium Citrate pH6
Permeabilization
No
Specification
colon
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Fixative
10% Normal buffered formalin
Username

Abcam user community

Verified customer

Submitted Mar 17 2017

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (small intestine)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: 10mM citrate buffer pH6
Permeabilization
No
Specification
small intestine
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Fixative
10% normal buffered formalin
Username

Abcam user community

Verified customer

Submitted Mar 17 2017

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Rat Tissue sections (cancerous liver)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: 10mM Citrate pH6
Permeabilization
No
Specification
cancerous liver
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Fixative
Paraformaldehyde
Username

Abcam user community

Verified customer

Submitted Mar 17 2017

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (beta cells)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: 10mm sodium citrate PH 6
Permeabilization
No
Specification
beta cells
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Fixative
Paraformaldehyde
Username

Abcam user community

Verified customer

Submitted Feb 07 2017

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Dog Tissue sections (pancreas)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: CC1
Permeabilization
No
Specification
pancreas
Fixative
formalin 4%
Username

Dr. Emmanuel Bouchaert

Verified customer

Submitted Jan 06 2017

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Pancreas)
Antigen retrieval step
None
Permeabilization
No
Specification
Pancreas
Blocking step
Serum Free Protein Block (Dako) as blocking agent for 5 minute(s) · Concentration: 100% · Temperature: 24°C
Fixative
10% NBF
Username

Premier Laboratory

Verified customer

Submitted May 04 2016

We recommend ab25693. This is currently the only chromogranin antibody for which we have canine IHC data. A collaborator tested it on sections of formalin-fixed paraffin-embedded canine small intestine and saw strong positive staining of a sub-populati...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Colon)
Specification
Colon
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: 10 mM Citrate
Permeabilization
No
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Feb 08 2013

Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products.
I am sorry these 2 products did not perform as stated on the datasheet and for the inconvenience this h...

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