Anti-Chromogranin A antibody [EP1030Y] (ab68271)

Overview

  • Product nameAnti-Chromogranin A antibody [EP1030Y]
    See all Chromogranin A primary antibodies
  • Description
    Rabbit monoclonal [EP1030Y] to Chromogranin A
  • Tested applicationsSuitable for: WB, IHC-P, ICC, Flow Cytmore details
    Unsuitable for: IP
  • Species reactivity
    Reacts with: Human
  • Immunogen

    A synthetic peptide corresponding to residues near the N-terminus of human Chromogranin A.

  • Positive control
    • SH-SY5Y cell lysate and human pancreas tissue.
  • General notes

    A trial size is available to purchase for this antibody.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Produced using Abcam's RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5, 675, 063 and/or 7, 429, 487.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab68271 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/10000 - 1/50000. Detects a band of approximately 90 kDa (predicted molecular weight: 51 kDa).
IHC-P 1/100 - 1/250.
ICC 1/100 - 1/250.
Flow Cyt 1/20.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

  • Application notesIs unsuitable for IP.
  • Target

    • FunctionPancreastatin strongly inhibits glucose induced insulin release from the pancreas.
    • Sequence similaritiesBelongs to the chromogranin/secretogranin protein family.
    • Post-translational
      modifications
      Sulfated on tyrosine residues and/or contains sulfated glycans.
      O-glycosylated with core 1 or possibly core 8 glycans.
    • Cellular localizationSecreted. Neuroendocrine and endocrine secretory granules.
    • Information by UniProt
    • Database links
    • FormAccording to the Swiss-Prot database, this protein has many different forms which correspond to various molecular weights; thus the possible variation from the predicted molecular weight of 51kDa.
    • Alternative names
      • beta Granin antibody
      • betagranin (N-terminal fragment of chromogranin A) antibody
      • catestatin antibody
      • CgA antibody
      • CHG A antibody
      • Chga antibody
      • chromofungin antibody
      • Chromogranin A parathyroid secretory protein 1 antibody
      • Chromogranin A precursor antibody
      • ChromograninA antibody
      • CMGA_HUMAN antibody
      • ER-37 antibody
      • Pancreastatin antibody
      • Parastatin antibody
      • Parathyroid secretory protein 1 antibody
      • Pituitary secretory protein I antibody
      • Secretory protein I antibody
      • SP I antibody
      • SP-I antibody
      • SP1 antibody
      • SPI antibody
      • Vasostatin antibody
      • Vasostatin I antibody
      • Vasostatin II antibody
      see all

    Anti-Chromogranin A antibody [EP1030Y] images

    • Anti-Chromogranin A antibody [EP1030Y] (ab68271) + SH-SY5Y cell lysate at 10 µg

      Secondary
      HRP conjugated Goat anti-rabbit at 1/2000 dilution

      Predicted band size : 51 kDa
      Observed band size : 90 kDa (why is the actual band size different from the predicted?)
    • Human pancreas tissue stained with ab68271 at 1/100 dilution.

    References for Anti-Chromogranin A antibody [EP1030Y] (ab68271)

    ab68271 has not yet been referenced specifically in any publications.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"