Anti-Chromogranin A antibody [EP1031Y] (ab52983)

Overview

  • Product nameAnti-Chromogranin A antibody [EP1031Y]
    See all Chromogranin A primary antibodies
  • Description
    Rabbit monoclonal [EP1031Y] to Chromogranin A
  • Tested applicationsSuitable for: WB, IP, IHC-Pmore details
    Unsuitable for: Flow Cyt or ICC
  • Species reactivity
    Reacts with: Rat, Human
  • Immunogen

    A synthetic peptide corresponding to residues near the C-terminus of human Chromogranin A was used as an immunogen.

  • Positive control
    • PC12 cytoplasmic cell lysate Human pancreatic tissue
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

     

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    A trial size is available to purchase for this antibody.

    Mouse: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab52983 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1e+006. Detects a band of approximately 80-90 kDa (predicted molecular weight: 51 kDa).Can be blocked with Chromogranin A peptide (ab138920).
IP 1/30.
IHC-P 1/250 - 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
  • Application notesIs unsuitable for Flow Cyt or ICC.
  • Target

    • FunctionPancreastatin strongly inhibits glucose induced insulin release from the pancreas.
    • Sequence similaritiesBelongs to the chromogranin/secretogranin protein family.
    • Post-translational
      modifications
      Sulfated on tyrosine residues and/or contains sulfated glycans.
      O-glycosylated with core 1 or possibly core 8 glycans.
    • Cellular localizationSecreted. Neuroendocrine and endocrine secretory granules.
    • Information by UniProt
    • Database links
    • FormAccording to the Swiss-Prot database, this protein has many different forms which correspond to various molecular weights; thus the possible variation from the predicted molecular weight of 51kDa.
    • Alternative names
      • beta Granin antibody
      • betagranin (N-terminal fragment of chromogranin A) antibody
      • catestatin antibody
      • CgA antibody
      • CHG A antibody
      • Chga antibody
      • chromofungin antibody
      • Chromogranin A parathyroid secretory protein 1 antibody
      • Chromogranin A precursor antibody
      • ChromograninA antibody
      • CMGA_HUMAN antibody
      • ER-37 antibody
      • Pancreastatin antibody
      • Parastatin antibody
      • Parathyroid secretory protein 1 antibody
      • Pituitary secretory protein I antibody
      • Secretory protein I antibody
      • SP I antibody
      • SP-I antibody
      • SP1 antibody
      • SPI antibody
      • Vasostatin antibody
      • Vasostatin I antibody
      • Vasostatin II antibody
      see all

    Anti-Chromogranin A antibody [EP1031Y] images

    • Anti-Chromogranin A antibody [EP1031Y] (ab52983) at 1/1000000 dilution + PC12 cell lysate at 10 µg

      Secondary
      goat anti-rabbit HRP labelled at 1/2000 dilution

      Predicted band size : 51 kDa
      Observed band size : 80-90 kDa (why is the actual band size different from the predicted?)
    • ab52983, at a 1/250 dilution, staining Human Chromogranin A in Pancreas using Immunohistochemistry, Paraffin Emebedded tissue.
    • Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD

    References for Anti-Chromogranin A antibody [EP1031Y] (ab52983)

    ab52983 has not yet been referenced specifically in any publications.

    Product Wall

    Thank you for contacting us.

    As requested, the blocking peptide for the Chromogranin A antibody ab52983 has been added to our catalogue. The catalogue reference number is ab138920 and the link to the datasheet is http://www.abcam.com/ab138920....

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"