Anti-Chromogranin A antibody [LK2H10 + PHE5] (ab715)

Overview

  • Product nameAnti-Chromogranin A antibody [LK2H10 + PHE5]
    See all Chromogranin A primary antibodies
  • Description
    Mouse monoclonal [LK2H10 + PHE5] to Chromogranin A
  • SpecificityThis antibody recognizes a protein of 68-75 kDa, identified as chromogranin A (a protein of 439-amino acid which is encoded on chromosome 14).
  • Tested applicationsICC/IF, IHC-P, IHC-Frmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human, Monkey
  • Immunogen

    Human pheochromocytoma

  • EpitopeAlthough the epitopes for these two clones are not precisely mapped, experimental data suggest that they are different.
  • Positive control
    • Pancreas, adrenal gland, bowel and thyroid
  • General notesThis antibody is a mixture of two clones, LK2H10 and PHE5.

Properties

Applications

Our Abpromise guarantee covers the use of ab715 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent dilution.
IHC-P 1/50 - 1/100.
IHC-Fr Use at an assay dependent dilution.

Target

  • FunctionPancreastatin strongly inhibits glucose induced insulin release from the pancreas.
  • Sequence similaritiesBelongs to the chromogranin/secretogranin protein family.
  • Post-translational
    modifications
    Sulfated on tyrosine residues and/or contains sulfated glycans.
    O-glycosylated with core 1 or possibly core 8 glycans.
  • Cellular localizationSecreted. Neuroendocrine and endocrine secretory granules.
  • Information by UniProt
  • Database links
  • FormAccording to the Swiss-Prot database, this protein has many different forms which correspond to various molecular weights; thus the possible variation from the predicted molecular weight of 51kDa.
  • Alternative names
    • beta Granin antibody
    • CGA antibody
    • CHG A antibody
    • CHGA antibody
    • Chromogranin A parathyroid secretory protein 1 antibody
    • Chromogranin A precursor antibody
    • ChromograninA antibody
    • CMGA_HUMAN antibody
    • ER-37 antibody
    • Pancreastatin antibody
    • Parastatin antibody
    • Parathyroid secretory protein 1 antibody
    • Pituitary secretory protein I antibody
    • SP I antibody
    • SP-I antibody
    • SP1 antibody
    • SPI antibody
    • Vasostatin antibody
    • Vasostatin I antibody
    • Vasostatin II antibody
    see all

References for Anti-Chromogranin A antibody [LK2H10 + PHE5] (ab715)

This product has been referenced in:
  • Burke AP  et al. Carcinoids of the jejunum and ileum: an immunohistochemical and clinicopathologic study of 167 cases. Cancer 79:1086-93 (1997). Read more (PubMed: 9070484) »
  • Angelsen A  et al. Neuroendocrine differentiation in carcinomas of the prostate: do neuroendocrine serum markers reflect immunohistochemical findings? Prostate 30:1-6 (1997). Read more (PubMed: 9018329) »

See all 8 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (Prostate)
Specification Prostate
Fixative Paraformaldehyde
Permeabilization Yes - 0.8% Triton X-100
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 20% · Temperature: 24°C
Username

Abcam user community

Verified customer

Submitted Jan 04 2008

Thank you for your enquiry and patience. I have confirmed with the laboratory that the ab715 antibody's concentration is 200ug/ml and the purification is by protein G chromatography. I hope this information will be useful to you. If you require a...

Read More

Thank you for your enquiry. Unfortunately, we do not have more information about this antibody. We will update the on-line datasheet of this product as soon as we get more data. We apologize for any inconvenience caused. If you have any questions in th...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"