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Our Abpromise guarantee covers the use of ab715 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use at an assay dependent concentration.|
|IHC-P||1/50 - 1/100.|
|IHC-Fr||Use at an assay dependent concentration.|
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of neuroendocrine tumor tissue labeling Chromogranin A with ab715.
Immunocytochemistry/ Immunofluorescence analysis of human prostate cells labeling Chromogranin A with ab715 at 1/200 dilution (green). Cells were fixed with 4% paraformaldehyde and permeabilized with 0.8% Triton X-100. A donkey anti-mouse (Alexa Fluor® 647) was used as the secondary antibody at 1/400 dilution.