Anti-Chromogranin A antibody [LK2H10], prediluted (ab8204)


  • Product nameAnti-Chromogranin A antibody [LK2H10], prediluted
    See all Chromogranin A primary antibodies
  • Description
    Mouse monoclonal [LK2H10] to Chromogranin A, prediluted
  • SpecificityThe antibody is specific for chromogranin A. Specificity was ascertained by immunoblotting and immunohistochemistry. The antibody predominantly reacts with a 68 kDa protein.
  • Tested applicationsSuitable for: WB, IHC-FoFr, IHC-Fr, IHC-Pmore details
  • Species reactivity
    Reacts with: Human, Pig
  • Immunogen

    Antigen was isolated from human Pheochromocytoma.

  • EpitopeHuman. Crossreactivity with monkey and pig.
  • Positive control
    • Adrenal medulla or pancreas tissue.



Our Abpromise guarantee covers the use of ab8204 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. PubMed: 19041781
IHC-FoFr 1/10.
IHC-Fr 1/10. Acetone fixed.
IHC-P 1/10.


  • FunctionPancreastatin strongly inhibits glucose induced insulin release from the pancreas.
  • Sequence similaritiesBelongs to the chromogranin/secretogranin protein family.
  • Post-translational
    Sulfated on tyrosine residues and/or contains sulfated glycans.
    O-glycosylated with core 1 or possibly core 8 glycans.
  • Cellular localizationSecreted. Neuroendocrine and endocrine secretory granules.
  • Information by UniProt
  • Database links
  • FormAccording to the Swiss-Prot database, this protein has many different forms which correspond to various molecular weights; thus the possible variation from the predicted molecular weight of 51kDa.
  • Alternative names
    • beta Granin antibody
    • CgA antibody
    • CHG A antibody
    • Chga antibody
    • Chromogranin A parathyroid secretory protein 1 antibody
    • Chromogranin A precursor antibody
    • ChromograninA antibody
    • CMGA_HUMAN antibody
    • ER-37 antibody
    • Pancreastatin antibody
    • Parastatin antibody
    • Parathyroid secretory protein 1 antibody
    • Pituitary secretory protein I antibody
    • SP I antibody
    • SP-I antibody
    • SP1 antibody
    • SPI antibody
    • Vasostatin antibody
    • Vasostatin I antibody
    • Vasostatin II antibody
    see all

Anti-Chromogranin A antibody [LK2H10], prediluted images

  • ab8204 staining Chromogen A in normal Human colon mucosa tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 1% BSA for 30 minutes at room temperature; antigen retrieval was by heat mediation with citrate buffer. Samples were incubated with primary antibody (1/10 in TBS) for 1 hour. An undiluted HRP-conjugated Goat polyclonal was used as the secondary antibody.

    See Abreview

References for Anti-Chromogranin A antibody [LK2H10], prediluted (ab8204)

This product has been referenced in:
  • Moran AW  et al. Sweet taste receptor expression in ruminant intestine and its activation by artificial sweeteners to regulate glucose absorption. J Dairy Sci 97:4955-72 (2014). IHC-Fr ; Sheep, Cow . Read more (PubMed: 24881785) »
  • Moran AW  et al. Expression of Na+/glucose co-transporter 1 (SGLT1) is enhanced by supplementation of the diet of weaning piglets with artificial sweeteners. Br J Nutr 104:637-46 (2010). IHC-Fr ; Pig . Read more (PubMed: 20338074) »

See all 6 Publications for this product

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (colon normal mucosa)
Specification colon normal mucosa
Fixative Formaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: citrate
Permeabilization No
Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: RT°C

Dr. Karin Birkenkamp-Demtroeder

Verified customer

Submitted Aug 13 2010

Thank you for your enquiry. The concentration of this antibody is 18 ug/ml. In addition, I noticed a mistake on the datasheet. The antibody is not purified. I have updated the datasheet to reflect this and have included a link for your convenience...

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Thank you for your enquiry. For ab8204, the concentration is 15-16 ug/ml. For ab8205, we are sorry to inform you that there is no protein concentration for polyclonal antibodies that are not affinity purified.This antibody is provided as whole serum an...

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The concentration is 15-16 microgram/ml. The product has a four day lead time so should be in stock four days from when the order is placed.