Overview

  • Product name
    Anti-Chromogranin B antibody
    See all Chromogranin B primary antibodies
  • Description
    Rabbit polyclonal to Chromogranin B
  • Host species
    Rabbit
  • Specificity
    Ab12242 recognises chromogranin B.
  • Tested applications
    Suitable for: WB, ICC/IF, IHC-Fr, ICC, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    C-terminal 15 amino acids of chromogranin B conjugated to ovalbumin.

Properties

Applications

Our Abpromise guarantee covers the use of ab12242 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/100 - 1/500. Predicted molecular weight: 78.2 kDa.
ICC/IF Use at an assay dependent concentration. PubMed: 24454876
IHC-Fr 1/100 - 1/500.
ICC Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration.

Target

  • Function
    Secretogranin-1 is a neuroendocrine secretory granule protein, which may be the precursor for other biologically active peptides.
  • Tissue specificity
    Expressed in the adrenal medulla, and in pheochromocytoma. Not expressed in liver.
  • Sequence similarities
    Belongs to the chromogranin/secretogranin protein family.
  • Post-translational
    modifications
    Extensively processed by limited proteolysis at conserved basic residues. Alternative processing are seen in different tissues.
  • Cellular localization
    Secreted. Neuroendocrine and endocrine secretory granules.
  • Information by UniProt
  • Database links
  • Alternative names
    • CCB peptide antibody
    • Cg B antibody
    • CgB antibody
    • Chgb antibody
    • Chromogranin-B antibody
    • ChromograninB antibody
    • SCG1 antibody
    • SCG1_HUMAN antibody
    • Secretogranin 1 antibody
    • Secretogranin B antibody
    • Secretogranin I antibody
    • SgI antibody
    see all

Images

  • ab12242(1:1000) staining Chromogranin B in human pancreas (IgG inset) using an automated system (DAKO Autostainer Plus). Using this protocol there is strong staining of Chromogranin B in secretory compartments of the pancreas.
    Insert panel depicts negative control (no primary antibody).
    Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffer EDTA pH9.0 in a DAKO PT link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX.
    Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required.

References

This product has been referenced in:
  • Stephens SB  et al. The Prohormone VGF Regulates ß Cell Function via Insulin Secretory Granule Biogenesis. Cell Rep 20:2480-2489 (2017). WB, ICC/IF . Read more (PubMed: 28877479) »
  • Wang H  et al. RanBP9 overexpression accelerates loss of pre and postsynaptic proteins in the AP?E9 transgenic mouse brain. PLoS One 9:e85484 (2014). WB, ICC/IF ; Mouse . Read more (PubMed: 24454876) »

See all 6 Publications for this product

Customer reviews and Q&As

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Tissue lysate - other (Cerebrospinal fluid (CSF))
Loading amount
3 µg
Specification
Cerebrospinal fluid (CSF)
Gel Running Conditions
Non-reduced Denaturing (12% SDS)
Blocking step
Milk as blocking agent for 17 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Username

Ms. Christiane Rewerts

Verified customer

Submitted May 29 2009

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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