Our Abpromise guarantee covers the use of ab20245 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IP||Use at an assay dependent concentration.|
|Flow Cyt||Use at an assay dependent concentration. ab170192-Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody.|
|IHC-Fr||Use at an assay dependent concentration.|
|WB||Use at an assay dependent concentration. Predicted molecular weight: 71 kDa.|
|ICC/IF||Use at an assay dependent concentration.|
|ICC||Use a concentration of 2 µg/ml.|
|IHC-P||Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
Confocal image of immunofluorescence staining with Mouse monoclonal [SGII 6B1/3] to Chromogranin C;(ab20245; 2µg/ml for 1h at room temperature) in red and nuclear counterstain in blue on Rat Cell (Hippocampal neuron culture). Cells were fixed with paraformaldehyde; BSA (5%) was used as a blocking agent (40 minutes at room temperature). Secondary antibody was Goat anti-mouse IgG2b Alexa Fluor 568 Conjugation (1/600).