Overview

  • Product name
  • Description
    Rabbit polyclonal to CHSS2
  • Specificity
    This antibody detects endogenous levels of total CHSS2 protein.
  • Tested applications
    Suitable for: WB, ELISA, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    A synthesized peptide derived from the internal region of human CHSS2.

  • Positive control
    • IHC-P: Human cervix carcinoma tissue. WB: MCF-7 cells and extracts from LOVO cells and K562 cells.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.02% Sodium Azide
    Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    The antibody was affinity purified from rabbit antiserum by affinity chromatography using epitope specific immunogen.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab75052 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Predicted molecular weight: 85 kDa.
ELISA 1/40000.
IHC-P 1/50 - 1/100.
ICC/IF 1/500 - 1/1000.

Target

  • Relevance
    CHSS2 belongs to the chondroitin N-acetylgalactosaminyltransferase family. It has both beta-1,3-glucuronic acid and beta-1,4-N-acetylgalactosamine transferase activity and transfers glucuronic acid (GlcUA) from UDP-GlcUA and N-acetylgalactosamine (GalNAc) from UDP-GalNAc to the non-reducing end of the elongating chondroitin polymer. CHSS2 is ubiquitous; highly expressed in pancreas, ovary, brain, heart, skeletal muscle, colon, kidney, liver, stomach, small intestine and placenta.
  • Cellular localization
    Golgi apparatus, golgi stack membrane; Single pass type II membrane protein.
  • Database links
  • Alternative names
    • Chondroitin glucuronyltransferase II antibody
    • Chondroitin polymerizing factor antibody
    • Chondroitin sulfate synthase 2 antibody
    • CHPF antibody
    • CHSY2 antibody
    • CSS2 antibody
    • Glucuronosyl N acetylgalactosaminyl proteoglycan 4 beta N acetylgalactosaminyltransferase II antibody
    • N acetylgalactosaminyl proteoglycan 3 beta glucuronosyltransferase II antibody
    see all

Images

  • All lanes : Anti-CHSS2 antibody (ab75052) at 1/500 dilution

    Lane 1 : Extracts from LOVO cells
    Lane 2 : Extracts from K562 cells
    Lane 3 : Extracts from LOVO cells plus 5µg immunizing peptide

    Lysates/proteins at 5 µg per lane.


    Predicted band size : 85 kDa
    Observed band size : 85 kDa
  • Immunofluorescence analysis of CHSS2 in MCF-7 cells, using ab75052 at a 1/500 dilution.
    Left image: untreated.
    Right image: treated with immunizing peptide.
  • Immunohistochemical analysis of CHSS2 in paraffin embedded human cervix carcinoma tissue, using ab75052 at a 1/50 dilution.
    Left image: untreated.
    Right image: treated with immunizing peptide.

References

ab75052 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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