Overview

  • Product name
  • Description
    Rabbit polyclonal to CHST2
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog
  • Immunogen

    Synthetic peptide, corresponding to internal sequence amino acids 480-529 of Human CHST2

  • Positive control
    • 293T cell lysate.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer
    Preservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab84354 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 58 kDa (predicted molecular weight: 58 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

  • Function
    Catalyzes the transfer of sulfate to position 6 of non-reducing N-acetylglucosamine (GlcNAc) residues within keratan-like structures on N-linked glycans and within mucin-associated glycans that can ultimately serve as L-selectin ligands. L-selectin ligands are present in high endothelial cells (HEVs) and play a central role in lymphocyte homing at sites of inflammation. Participates in biosynthesis of L-selectin ligand sialyl 6-sulfo Lewis X and in lymphocyte homing to Peyer patches. Has no activity toward O-linked sugars. Its substrate specificity may be influenced by its subcellular location. Sulfates GlcNAc residues at terminal, non-reducing ends of oligosaccharide chains.
  • Tissue specificity
    Widely expressed. Highly expressed in bone marrow, peripheral blood leukocytes, spleen, brain, spinal cord, ovary and placenta. Expressed by high endothelial cells (HEVs) and leukocytes.
  • Sequence similarities
    Belongs to the sulfotransferase 1 family. Gal/GlcNAc/GalNAc subfamily.
  • Cellular localization
    Golgi apparatus > trans-Golgi network membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • AI428561 antibody
    • AW121776 antibody
    • C130041E03Rik antibody
    • C6ST antibody
    • Carbohydrate (chondroitin 6/keratan) sulfotransferase 2 antibody
    • Carbohydrate (N-acetylglucosamine-6-O) sulfotransferase 2 antibody
    • Carbohydrate sulfotransferase 2 antibody
    • CHST2 antibody
    • CHST2_HUMAN antibody
    • Chts2 antibody
    • Galactose/N-acetylglucosamine/N-acetylglucosamine 6-O-sulfotransferase 2 antibody
    • GlcNAc6ST antibody
    • GlcNAc6ST-1 antibody
    • Gn6st antibody
    • Gn6st-1 antibody
    • GST 2 antibody
    • GST-2 antibody
    • GST2 antibody
    • N-acetylglucosamine 6-O-sulfotransferase 1 antibody
    • N-acetylglucosamine 6-O-sulfotransferase antibody
    see all

Images

  • Anti-CHST2 antibody (ab84354) at 1 µg/ml + 293T cell lysate at 10 µg

    Secondary
    anti-Rabbit IgG HRP at 1/50000 dilution

    Predicted band size : 58 kDa
    Observed band size : 58 kDa
    Additional bands at : 31 kDa,92 kDa. We are unsure as to the identity of these extra bands.

References

ab84354 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab84354.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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