Recombinant
RabMAb

Anti-CHST3 antibody [EPR15789] (ab193359)

Overview

  • Product name
    Anti-CHST3 antibody [EPR15789]
  • Description
    Rabbit monoclonal [EPR15789] to CHST3
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, Flow Cytmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human CHST3 aa 400 to the C-terminus. The exact sequence is proprietary.
    Database link: Q7LGC8

  • Positive control
    • 293, HeLa, HepG2, Jurkat and Raw264.7 cell lysates; Jurkat cells.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab193359 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/2000. Detects a band of approximately 55 kDa (predicted molecular weight: 55 kDa).
Flow Cyt 1/350.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

Target

  • Function
    Catalyzes the transfer of sulfate to position 6 of the N-acetylgalactosamine (GalNAc) residue of chondroitin. Chondroitin sulfate constitutes the predominant proteoglycan present in cartilage and is distributed on the surfaces of many cells and extracellular matrices. Can also sulfate Gal residues of keratan sulfate, another glycosaminoglycan, and the Gal residues in sialyl N-acetyllactosamine (sialyl LacNAc) oligosaccharides. May play a role in the maintenance of naive T-lymphocytes in the spleen.
  • Tissue specificity
    Widely expressed in adult tissues. Expressed in heart, placenta, skeletal muscle and pancreas. Also expressed in various immune tissues such as spleen, lymph node, thymus and appendix.
  • Involvement in disease
    Defects in CHST3 are a cause of spondyloepiphyseal dysplasia with congenital joint dislocations (SEDC-JD) [MIM:143095]. A bone dysplasia clinically characterized by dislocation of the knees and/or hips at birth, clubfoot, elbow joint dysplasia with subluxation and limited extension, short stature, and progressive kyphosis developing in late childhood. The disorder is usually evident at birth, with short stature and multiple joint dislocations or subluxations that dominate the neonatal clinical and radiographic picture. During childhood, the dislocations improve, both spontaneously and with surgical treatment, and features of spondyloepiphyseal dysplasia become apparent, leading to arthritis of the hips and spine with intervertebral disk degeneration, rigid kyphoscoliosis, and trunk shortening by late childhood.
  • Sequence similarities
    Belongs to the sulfotransferase 1 family. Gal/GlcNAc/GalNAc subfamily.
  • Cellular localization
    Golgi apparatus membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • C6ST 1 antibody
    • C6ST antibody
    • C6ST-1 antibody
    • C6ST1 antibody
    • Carbohydrate (chondroitin 6) sulfotransferase 3 antibody
    • Carbohydrate sulfotransferase 3 antibody
    • Chondroitin 6 O sulfotransferase 1 antibody
    • Chondroitin 6 sulfotransferase antibody
    • Chondroitin 6-O-sulfotransferase 1 antibody
    • Chondroitin 6-sulfotransferase antibody
    • CHST 3 antibody
    • Chst3 antibody
    • CHST3_HUMAN antibody
    • Galactose/N acetylglucosamine/N acetylglucosamine 6 O sulfotransferase 0 antibody
    • Galactose/N-acetylglucosamine/N-acetylglucosamine 6-O-sulfotransferase 0 antibody
    • GST 0 antibody
    • GST-0 antibody
    • GST0 antibody
    see all

Images

  • All lanes : Anti-CHST3 antibody [EPR15789] (ab193359) at 1/1000 dilution

    Lane 1 : 293 cell lysate
    Lane 2 : HeLa cell lysate
    Lane 3 : HepG2 cell lysate
    Lane 4 : Jurkat cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 55 kDa
    Observed band size: 55 kDa



    Blocking/Dilution buffer: 5% NFDM/TBST.

  • Anti-CHST3 antibody [EPR15789] (ab193359) at 1/1000 dilution + Raw264.7 cell lysate at 10 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 55 kDa
    Observed band size: 55 kDa



    Blocking/Dilution buffer: 5% NFDM/TBST.

  • Flow cytometric analysis of 2% paraformaldehyde-fixed Jurkat cells labeling CHST3 with ab193359 at 1/350 dilution (red) compared with a rabbit monoclonal IgG isotype control (black) and a unlabelled control (cells without incubation with primary antibody and secondary antibody; blue). Goat anti rabbit IgG (FITC) at 1/150 dilution was used as the secondary antibody.

References

ab193359 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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