Overview

  • Product name
  • Description
    Rabbit polyclonal to CHST6
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Cow
  • Immunogen

    Recombinant fragment, corresponding to a region within amino acids 57-340 of Human CHST6 (Uniprot ID: Q9GZX3).

  • Positive control
    • HeLa, 293T, A431, H1299, HepG2, MOLT4 and Raji whole cell lysates.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    pH: 7.00
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 0.75% Glycine, 1.21% Tris, 10% Glycerol
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab154332 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 44 kDa.

Target

  • Function
    Sulfotransferase that utilizes 3'-phospho-5'-adenylyl sulfate (PAPS) as sulfonate donor to catalyze the transfer of sulfate to position 6 of non-reducing N-acetylglucosamine (GlcNAc) residues of keratan. Mediates sulfation of keratan in cornea. Keratan sulfate plays a central role in maintaining corneal transparency. Acts on the non-reducing terminal GlcNAc of short and long carbohydrate substrates that have poly-N-acetyllactosamine structures.
  • Tissue specificity
    Expressed in cornea. Mainly expressed in brain. Also expressed in spinal cord and trachea.
  • Involvement in disease
    Defects in CHST6 are the cause of macular dystrophy, corneal, 1 (MCDC1) [MIM:217800]. An ocular disease characterized by bilateral, progressive corneal opacification, and reduced corneal sensitivity. Onset occurs in the first decade, usually between ages 5 and 9. Painful attacks with photophobia, foreign body sensations, and recurrent erosions occur in most patients. The disease is due to deposition of an unsulfated keratan sulfate both within the intracellular space (within the keratocytes and endothelial cells) and in the extracellular corneal stroma. Macular corneal dystrophy is divided into the clinically indistinguishable types I, IA, and II based on analysis of the normally sulfated, or antigenic, keratan sulfate levels in serum and immunohistochemical evaluation of the cornea. Patients with types I and IA macular corneal dystrophy have undetectable serum levels of antigenic keratan sulfate, whereas those with type II macular corneal dystrophy have normal or low levels, depending on the population examined. Note=CHST6 homozygous missense mutations have been observed in patients with macular corneal dystrophy type I, while type II patients show a large deletion and replacement in the upstream region of CHST6. The only missense mutation for type II is Cys-50, which is heterozygous with a replacement in the upstream region on the other allele of CHST6.
  • Sequence similarities
    Belongs to the sulfotransferase 1 family. Gal/GlcNAc/GalNAc subfamily.
  • Cellular localization
    Golgi apparatus membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • C GlcNAc6ST antibody
    • C-GlcNAc6ST antibody
    • Carbohydate sulfotransferase 6 antibody
    • Carbohydrate (N acetylglucosamine 6 O) sulfotransferase 6 antibody
    • Carbohydrate sulfotransferase 6 antibody
    • CHST6 antibody
    • CHST6_HUMAN antibody
    • Corneal GlcNAc6-sulfotransferase antibody
    • Corneal N acetylglucosamine 6 sulfotransferase antibody
    • Corneal N-acetylglucosamine-6-O-sulfotransferase antibody
    • Galactose N acetylglucosamine N acetylglucosamine 6 O sulfotransferase 4 beta antibody
    • Galactose/N-acetylglucosamine/N-acetylglucosamine 6-O-sulfotransferase 4-beta antibody
    • GlcNAc6ST 5 antibody
    • GlcNAc6ST-5 antibody
    • Gn6st-5 antibody
    • GST4 beta antibody
    • GST4-beta antibody
    • hCGn6ST antibody
    • N-acetylglucosamine 6-O-sulfotransferase 5 antibody
    see all

Images

  • Anti-CHST6 antibody (ab154332) at 1/1000 dilution + HeLa whole cell lysate. at 30 µg

    Predicted band size: 44 kDa



    10% SDS PAGE

References

This product has been referenced in:
  • Gouveia RM  et al. Controlling the 3D architecture of Self-Lifting Auto-generated Tissue Equivalents (SLATEs) for optimized corneal graft composition and stability. Biomaterials 121:205-219 (2017). IF ; Human . Read more (PubMed: 28092777) »
  • Abidin FZ  et al. Application of retinoic acid improves form and function of tissue engineered corneal construct. Organogenesis 11:122-36 (2015). WB . Read more (PubMed: 26496651) »

See all 2 Publications for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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