The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 0.25 µg/ml. Predicted molecular weight: 49 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
RelevanceCHST8, or Carbohydrate sulfotransferase 8, catalyzes the transfer of sulfate to position 4 of non-reducing N-acetylgalactosamine (GalNAc) residues in both N-glycans and O-glycans. It is required for biosynthesis of glycoprotein hormones lutropin and thyrotropin, by mediating sulfation of their carbohydrate structures. CHST8 is only active against terminal GalNAcbeta1 and GalNAcbeta and not toward chondroitin.
Cellular localizationGolgi apparatus membrane; Single-pass type II membrane protein.