The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Dot: Use at an assay dependent dilution.
IP: Use at 10µg/mg RIPA lysate of HeLa cells.
WB: 1/100. Detects a band of approximately 118 kDa (predicted molecular weight: 107 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Chromosome cohesion factor involved in sister chromatid cohesion and fidelity of chromosome transmission. Component of one of the cell nuclear antigen loader complexes, CTF18-replication factor C (CTF18-RFC), which consists of CTF18, CTF8, DCC1, RFC2, RFC3, RFC4 and RFC5. The CTF18-RFC complex binds to single-stranded and primed DNAs and has weak ATPase activity that is stimulated by the presence of primed DNA, replication protein A (RPA) and by proliferating cell nuclear antigen (PCNA). The CTF18-RFC complex catalyzes the ATP-dependent loading of PCNA onto primed and gapped DNA. It also interacts with and stimulates DNA polymerase POLH.
Belongs to the activator 1 small subunits family. CTF18 subfamily.
Nucleus. Associates with chromatin during S phase.