Overview

  • Product name
  • Description
    Rabbit polyclonal to CHTF18
  • Tested applications
    Suitable for: IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Rhesus monkey, Gorilla
  • Immunogen

    Synthetic peptide corresponding to a region between residue 25 and 75 of Human CHTF18 (NP_071375.1).

  • Positive control
    • Human breast carcinoma, stomach adenocarcinoma and testicular seminoma tissue.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.09% Sodium Azide
    Constituents: 0.1% BSA, Tris buffered saline
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab84889 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P
  • Application notes
    IHC-P: 1/100 - 1/500. Epitope exposure is recommended. Exposure with citrate buffer will enhance staining.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function
      Chromosome cohesion factor involved in sister chromatid cohesion and fidelity of chromosome transmission. Component of one of the cell nuclear antigen loader complexes, CTF18-replication factor C (CTF18-RFC), which consists of CTF18, CTF8, DCC1, RFC2, RFC3, RFC4 and RFC5. The CTF18-RFC complex binds to single-stranded and primed DNAs and has weak ATPase activity that is stimulated by the presence of primed DNA, replication protein A (RPA) and by proliferating cell nuclear antigen (PCNA). The CTF18-RFC complex catalyzes the ATP-dependent loading of PCNA onto primed and gapped DNA. It also interacts with and stimulates DNA polymerase POLH.
    • Sequence similarities
      Belongs to the activator 1 small subunits family. CTF18 subfamily.
    • Cellular localization
      Nucleus. Associates with chromatin during S phase.
    • Information by UniProt
    • Database links
    • Alternative names
      • 6030457M03Rik antibody
      • C16orf41 antibody
      • C321D2.2 antibody
      • C321D2.3 antibody
      • C321D2.4 antibody
      • CHL12 antibody
      • chromosome transmission fidelity factor 18 homolog (S. cerevisiae) antibody
      • Chromosome transmission fidelity protein 18 homolog antibody
      • chtf18 antibody
      • Ctf18 antibody
      • CTF18_HUMAN antibody
      • hCTF18 antibody
      • MGC38008 antibody
      • RUVBL antibody
      see all

    Anti-CHTF18 antibody images

    • ab84889, at 1/250 dilution, staining CHTF18 in formalin-fixed, paraffin-embedded human breast carcinoma by Immunohistochemistry, using DAB detection.
    • ab84889, at 1/250 dilution, staining CHTF18 in formalin-fixed, paraffin-embedded human testicular seminoma by Immunohistochemistry, using DAB detection.
    • ab84889, at 1/100 dilution, staining CHTF18 in formalin-fixed, paraffin-embedded human breast carcinoma by Immunohistochemistry using a Red-fluorescent goat anti-rabbit IgG highly cross-adsorbed Hilyte PlusTM 555 secondary antibody at a dilution of 1/100.

    References for Anti-CHTF18 antibody (ab84889)

    ab84889 has not yet been referenced specifically in any publications.

    Product Wall

    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (HeLa)
    Specification
    HeLa
    Fixative
    Paraformaldehyde
    Permeabilization
    Yes - 0.5% Triton X100
    Username

    Dr. Kirk Mcmanus

    Verified customer

    Submitted Oct 19 2010

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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