Overview

  • Product nameAnti-CHX10 antibody - ChIP Grade
    See all CHX10 primary antibodies
  • Description
    Sheep polyclonal to CHX10 - ChIP Grade
  • Tested applicationsSuitable for: WB, ChIP, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Cow, Human
  • Immunogen

    Recombinant fragment:

    EAAAEKPEGERQALPKLDKMEQDERGPDAQAAISQEELRENSIAVLRAKA QEHSTKVLGTVSGPDSLARSTEKPEEEEAMDEDRPAERLSPPQLEDMA

    , corresponding to C terminal amino acids 264-361 of Human CHX10.

  • Positive control
    • Rat or mouse retinal tissue lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab16141 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
ChIP
IHC-P
  • Application notesChIP: Use at an assay dependent dilution (PMID 14272592).
    IHC-P: 1/200 (PMID 18981228).
    WB: Use at a concentration of 0.5 - 1 µg/ml. Detects a band of approximately 46 kDa.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionPlays a significant role in the specification and morphogenesis of the sensory retina. May also participate in the development of the cells of the inner nuclear layer, particularly bipolar cells.
    • Tissue specificityAbundantly expressed in retinal neuroblasts during eye development and in the inner nuclear layer of the adult retina. Within this layer, expression is stronger in the outer margin where bipolar cells predominate.
    • Involvement in diseaseDefects in VSX2 are the cause of microphthalmia isolated type 2 (MCOP2) [MIM:610093]; also known as isolated clinical anophthalmia. Microphthalmia is a clinically heterogeneous disorder of eye formation, ranging from small size of a single eye to complete bilateral absence of ocular tissues. Ocular abnormalities like opacities of the cornea and lens, scaring of the retina and choroid, cataractand other abnormalities like cataract may also be present.
      Defects in VSX2 are the cause of microphthalmia with cataracts and iris abnormalities (MCOPCTI) [MIM:610092].
      Defects in VSX2 are the cause of microphthalmia isolated with coloboma type 3 (MCOPCB3) [MIM:610092]; also known as isolated colobomatous microphthalmia 3. Ocular colobomas are a set of malformations resulting from abnormal morphogenesis of the optic cup and stalk, and the fusion of the fetal fissure (optic fissure).
    • Sequence similaritiesBelongs to the paired homeobox family.
      Contains 1 CVC domain.
      Contains 1 homeobox DNA-binding domain.
    • Cellular localizationNucleus.
    • Information by UniProt
    • Database links
    • Alternative names
      • C elegans ceh 10 homeo domain containing homolog antibody
      • Ceh 10 homeo domain containing homolog (C. elegans) antibody
      • Ceh 10 homeo domain containing homolog antibody
      • Ceh 10 homeodomain containing homolog antibody
      • Ceh 10 homeodomain containing homolog (C. elegans) antibody
      • Ceh-10 homeodomain-containing homolog antibody
      • Ceh10 homeo domain containing homolog antibody
      • Ceh10 homeodomain containing homolog antibody
      • CHX 10 antibody
      • Homeobox protein CHX 10 antibody
      • Homeobox protein CHX10 antibody
      • HOX 10 antibody
      • HOX10 antibody
      • MCOP 2 antibody
      • MCOP2 antibody
      • MCOPCB 3 antibody
      • MCOPCB3 antibody
      • RET 1 antibody
      • RET1 antibody
      • Visual system homeobox 2 antibody
      • Vsx 2 antibody
      • Vsx2 antibody
      • VSX2_HUMAN antibody
      see all

    Anti-CHX10 antibody - ChIP Grade images

    • ab16141 staining CHX10 in Mouse E12.5 spinal cord tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde and blocked with 10% donkey serum + 1% BSA in PBS with 0.1% Triton X-100 for 1 hour at room temperature; antigen retrieval was by heat mediation in a 0.01M sodium-citrate buffer. Samples were incubated with primary antibody (1/500 in 1% BSA in PBS with 0.1% Trition X-100) for 16 hours. An Alexa Fluor® 488-conjugated Donkey anti-sheep IgG polyclonal (1/400) was used as the secondary antibody.

      See Abreview

    • Western blot analysis using ab16141 at 1 µg/ml on rat liver (A), rat retina tissue lysate (B), mouse liver (C) and mouse retina tissue lysate (D).

      Western blot analysis using ab16141 at 1 µg/ml on rat liver (A), rat retina tissue lysate (B), mouse liver (C) and mouse retina tissue lysate (D).

    References for Anti-CHX10 antibody - ChIP Grade (ab16141)

    This product has been referenced in:
    • Kanda A  et al. Atp6ap2/(pro)renin receptor interacts with Par3 as a cell polarity determinant required for laminar formation during retinal development in mice. J Neurosci 33:19341-51 (2013). Mouse . Read more (PubMed: 24305829) »
    • Chang HH  et al. Intravitreal homocysteine-thiolactone injection leads to the degeneration of multiple retinal cells, including photoreceptors. Mol Vis 17:1946-56 (2011). IHC-P ; Mouse . Read more (PubMed: 21850169) »

    See all 4 Publications for this product

    Product Wall

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Blocking step 10% donkey serum + 1% BSA in PBS with 0.1% Triton X-100 as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: RT°C
    Antigen retrieval step Heat mediated - Buffer/Enzyme Used: 0.01M sodium-citrate buffer pH 6.0
    Sample Mouse Tissue sections (E12.5 mouse spinal cord)
    Specification E12.5 mouse spinal cord
    Permeabilization Yes - 0.1% Triton-X 100
    Fixative Paraformaldehyde
    Username

    Abcam user community

    Verified customer

    Submitted Aug 06 2013

    Thank you very much for your interest in ab16141.

    To our knowledge, ab16141 has not been tested in IHC-Fr. Therefore, I can offer a discount off a future purchase if you buy ab16141 now, test it inIHC-Fr and submit feedback to us in the form...

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"