Anti-Chymotrypsin antibody (ab231724)
Key features and details
- Rabbit polyclonal to Chymotrypsin
- Suitable for: WB, IHC-P
- Reacts with: Rat
- Isotype: IgG
Overview
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Product name
Anti-Chymotrypsin antibody
See all Chymotrypsin primary antibodies -
Description
Rabbit polyclonal to Chymotrypsin -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Rat
Predicted to work with: Mouse -
Immunogen
Recombinant full length protein (His-T7-tag) corresponding to Rat Chymotrypsin aa 1 to the C-terminus. Mature chain lacking signal and propeptides. Expressed in E.coli. N-terminal tags.
Database link: P55091 -
Positive control
- WB: Recombinant rat Chymotrypsin; rat pancreas and testis lysates; IHC-P: Rat pancreas tissue.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.011% Proclin 300
Constituents: 55.77% Glycerol (glycerin, glycerine), 44.219% PBS -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
Antigen-specific affinity chromatography followed by Protein A affinity chromatography. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab231724 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use a concentration of 0.2 - 2 µg/ml. Predicted molecular weight: 29 kDa.
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IHC-P |
Use a concentration of 5 - 20 µg/ml.
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Notes |
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WB
Use a concentration of 0.2 - 2 µg/ml. Predicted molecular weight: 29 kDa. |
IHC-P
Use a concentration of 5 - 20 µg/ml. |
Target
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Function
Has chymotrypsin-type protease activity and hypocalcemic activity. -
Tissue specificity
Pancreas. -
Involvement in disease
Variations in CTRC influence susceptibility to chronic pancreatitis [MIM:167800]. Chronic pancreatitis is a persistent inflammatory disorder characterized by permanent destruction of the pancreatic parenchyma. -
Sequence similarities
Belongs to the peptidase S1 family. Elastase subfamily.
Contains 1 peptidase S1 domain. - Information by UniProt
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Database links
- Entrez Gene: 76701 Mouse
- Entrez Gene: 362653 Rat
- SwissProt: Q3SYP2 Mouse
- SwissProt: P55091 Rat
- Unigene: 440471 Mouse
- Unigene: 19214 Rat
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Alternative names
- Caldecrin antibody
- Chymotrypsin C antibody
- Chymotrypsin-C antibody
see all
Images
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Formalin-fixed, paraffin-embedded rat pancreas tissue stained for Chymotrypsin using ab231724 at 20 μg/ml in immunohistochemical analysis. DAB staining.
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Anti-Chymotrypsin antibody (ab231724) at 2 µg/ml + Rat pancreas lysate
Predicted band size: 29 kDa -
Anti-Chymotrypsin antibody (ab231724) at 2 µg/ml + Rat testis lysate
Predicted band size: 29 kDa -
Formalin-fixed, paraffin-embedded rat pancreas tissue stained for Chymotrypsin using ab231724 at 20 μg/ml in immunohistochemical analysis. DAB staining.
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Anti-Chymotrypsin antibody (ab231724) at 2 µg/ml + Recombinant rat Chymotrypsin protein
Predicted band size: 29 kDa
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab231724 has not yet been referenced specifically in any publications.