Overview

  • Product name
  • Description
    Rabbit polyclonal to CIAO1
  • Tested applications
    Suitable for: WB, ELISA, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Chicken, Cow, Cat, Dog, Pig, Saccharomyces cerevisiae
  • Immunogen

    Synthetic peptide corresponding to a region within internal sequence amino acids 143-192 (SVLNSHTQDV KHVVWHPSQE LLASASYDDT VKLYREEEDD WVCCATLEGH) of human CIA01 (NP_004795).

  • Positive control
    • 721_B cell lysate

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Storage buffer
    Preservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    Purified by peptide affinity chromatography method.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab83088 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 38 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA Use at an assay dependent concentration.

ELISA titre using peptide based assay, 1:62500.

IHC-P 1/100.

Target

Anti-CIAO1 antibody images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human lung tissue labelling CIAO1 with ab83088 at 1/100. A Cy3-conjugated donkey anti-rabbit IgG (1/200) was used as the secondary antibody. Positive staining shown in the cytoplasm of alveolar type I cells. Magnification: 20X. Exposure time: 0.5 - 2.0 seconds. Left - DAPI. Middle - CIAO1. Right - Merge.
  • Anti-CIAO1 antibody (ab83088) at 1 µg/ml + 712_B cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 38 kDa
    Observed band size : 38 kDa
    Additional bands at : 26 kDa,64 kDa. We are unsure as to the identity of these extra bands.


  • Predicted band size : 38 kDa
    Western Blot using ab83088 at 1.0ug/ml. Sample type: Human Hela cell Lysate


  • Predicted band size : 38 kDa
    Western Blot using ab83088 at 1.0ug/ml. Sample type: Human MCF7 cell Lysate

References for Anti-CIAO1 antibody (ab83088)

This product has been referenced in:
  • Odermatt DC & Gari K The CIA Targeting Complex Is Highly Regulated and Provides Two Distinct Binding Sites for Client Iron-Sulfur Proteins. Cell Rep 18:1434-1443 (2017). Read more (PubMed: 28178521) »

See 1 Publication for this product

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There are currently no Abreviews or Questions for ab83088.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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