Recombinant
RabMAb

Anti-cIAP1 antibody [EPR4673] (HRP) (ab207526)

Overview

  • Product name
    Anti-cIAP1 antibody [EPR4673] (HRP)
    See all cIAP1 primary antibodies
  • Description
    Rabbit monoclonal [EPR4673] to cIAP1 (HRP)
  • Conjugation
    HRP
  • Tested applications
    Suitable for: IHC-P, WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human cIAP1 aa 1-100. The exact sequence is proprietary.
    Database link: Q13490

  • Positive control
    • WB: HeLa and HepG2 whole cell lysates. IHC-P: FFPE human spleen (normal) tissue sections.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

Properties

Applications

Our Abpromise guarantee covers the use of ab207526 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/5000. Detects a band of approximately 75 kDa (predicted molecular weight: 70 kDa).

Target

  • Function
    Apoptotic suppressor. The BIR motifs region interacts with TNF receptor associated factors 1 and 2 (TRAF1 and TRAF2) to form an heteromeric complex, which is then recruited to the tumor necrosis factor receptor 2 (TNFR2).
  • Tissue specificity
    Present in many fetal and adult tissues. Mainly expressed in adult skeletal muscle, thymus, testis, ovary, and pancreas, low or absent in brain and peripheral blood leukocytes.
  • Sequence similarities
    Belongs to the IAP family.
    Contains 3 BIR repeats.
    Contains 1 CARD domain.
    Contains 1 RING-type zinc finger.
  • Cellular localization
    Cytoplasm.
  • Information by UniProt
  • Database links
  • Alternative names
    • API 1 antibody
    • API1 antibody
    • Apoptosis inhibitor 1 antibody
    • Baculoviral IAP repeat containing 2 antibody
    • Baculoviral IAP repeat containing protein 2 antibody
    • Baculoviral IAP repeat-containing protein 2 antibody
    • BIRC 2 antibody
    • BIRC2 antibody
    • BIRC2_HUMAN antibody
    • C IAP1 antibody
    • C-IAP1 antibody
    • cIAP 1 antibody
    • cIAP1 antibody
    • HIAP 2 antibody
    • HIAP-2 antibody
    • HIAP2 antibody
    • IAP 2 antibody
    • IAP homolog B antibody
    • IAP-2 antibody
    • IAP2 antibody
    • Inhibitor of apoptosis protein 2 antibody
    • MIHB antibody
    • NFR2 TRAF signalling complex protein antibody
    • RING finger protein 48 antibody
    • RNF 48 antibody
    • RNF48 antibody
    • TNFR2 TRAF signaling complex protein 2 antibody
    • TNFR2-TRAF-signaling complex protein 2 antibody
    see all

Images

  • All lanes : Anti-cIAP1 antibody [EPR4673] (HRP) (ab207526) at 1/5000 dilution

    Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 2 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 70 kDa
    Observed band size : 75 kDa (why is the actual band size different from the predicted?)


    Exposure time : 20 seconds

    This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab207526 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.

  • IHC image of cIAP1 staining in a section of formalin-fixed paraffin-embedded normal human spleen tissue performed on a Leica BONDTM. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20mins.  The section was then incubated with ab207526, 1/50 dilution, for 15 mins at room temperature. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset negative control image is taken from an identical assay without primary antibody.

     

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

     

    *Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre

References

ab207526 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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