Overview

  • Product nameAnti-cIAP2 antibody
    See all cIAP2 primary antibodies
  • Description
    Rabbit polyclonal to cIAP2
  • Tested applicationsSuitable for: WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Chicken, Dog
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 550 to the C-terminus of Human cIAP2.

    (Peptide available as ab25892.)

  • Positive control
    • This antibody gave a positive signal in HeLa cells.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab23423 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 71 kDa (predicted molecular weight: 68 kDa).
ICC/IF Use a concentration of 1 µg/ml.

Target

  • FunctionApoptotic suppressor. The BIR motifs region interacts with TNF receptor associated factors 1 and 2 (TRAF1 and TRAF2) to form an heteromeric complex, which is then recruited to the tumor necrosis factor receptor 2 (TNFR2).
  • Tissue specificityHighly expressed in fetal lung, and kidney. In the adult, expression is mainly seen in lymphoid tissues, including spleen, thymus and peripheral blood lymphocytes.
  • Involvement in diseaseNote=A chromosomal aberration involving BIRC3 is recurrent in low-grade mucosa-associated lymphoid tissue (MALT lymphoma). Translocation t(11;18)(q21;q21) with MALT1. This translocation is found in approximately 50% of cytogenetically abnormal low-grade MALT lymphoma.
  • Sequence similaritiesBelongs to the IAP family.
    Contains 3 BIR repeats.
    Contains 1 CARD domain.
    Contains 1 RING-type zinc finger.
  • Cellular localizationCytoplasm.
  • Information by UniProt
  • Database links
  • Alternative names
    • AIP 1 antibody
    • AIP1 antibody
    • API 2 antibody
    • API2 antibody
    • Apoptosis inhibitor 2 antibody
    • Baculoviral IAP repeat containing 3 antibody
    • Baculoviral IAP repeat containing protein 3 antibody
    • Baculoviral IAP repeat-containing protein 3 antibody
    • BIRC 3 antibody
    • BIRC3 antibody
    • BIRC3_HUMAN antibody
    • C IAP2 antibody
    • C-IAP2 antibody
    • CIAP 2 antibody
    • CIAP2 antibody
    • HAIP 1 antibody
    • HAIP1 antibody
    • HIAP 1 antibody
    • HIAP-1 antibody
    • HIAP1 antibody
    • IAP homolog C antibody
    • IAP-1 antibody
    • Inhibitor of apoptosis protein 1 antibody
    • MALT 2 antibody
    • MALT2 antibody
    • Mammalian IAP homolog C antibody
    • MIHC antibody
    • RING finger protein 49 antibody
    • RNF49 antibody
    • TNFR2 TRAF signaling complex protein 1 antibody
    • TNFR2 TRAF signalling complex protein antibody
    • TNFR2-TRAF-signaling complex protein 1 antibody
    see all

Anti-cIAP2 antibody images

  • ICC/IF image of ab23423 stained human HeLa cells. The cells were methanol fixed (5 min) and incubated with the antibody (ab23423, 1µg/ml) for 1h at room temperature. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Image-iTTM FX Signal Enhancer was used as the primary blocking agent, 5% BSA (in TBS-T) was used for all other blocking steps. DAPI was used to stain the cell nuclei (blue). Alexa Fluor® 594 WGA was used to label plasma membranes (red).

  • All lanes : Anti-cIAP2 antibody (ab23423) at 1 µg/ml

    Lane 1 : Daudi (Human Burkitt's lymphoma cell line) Whole Cell Lysate
    Lane 2 : MOLT4 (Human acute lymphoblastic leukemia cell line) Whole Cell Lysate
    Lane 3 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
    Lane 4 : Ramos (Human Burkitt's lymphoma cell line) Whole Cell Lysate
    Lane 5 : Raji (Human Burkitt's lymphoma cell line) Whole Cell Lysate
    Lane 6 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg/ml per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 68 kDa
    Observed band size : 73 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 150 kDa,35 kDa,75 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 8 minutes
    A doublet was observed in Daudi, Ramos and Raji whole cell lysates (lanes 1, 4 and 5). This data suggests that ab23423 might react with both cIAP1 and cIAP2 proteins. The predicted molecular weights of cIAP1 and cIAP2 are 69-kDa and 68-kDa respectively. Abcam welcomes customer feedback and would appreciate any comments regarding this product and the data presented above.
  • ab23423 staining cIAP2 in HeLa cells treated with TMCB (ab120289), by ICC/IF. Decrease in cIAP2 expression correlates with increased concentration of TMCB, as described in literature.
    The cells were incubated at 37°C for 10 minutes in media containing different concentrations of ab120289 (TMCB) in DMSO, fixed with 4% formaldehyde for 10 minutes at room temperature and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab23423 (5 µg/ml) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 goat anti-rabbit polyclonal antibody (ab96899) at 1/250 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI and are shown in blue.

  • Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 68 kDa

    HeLa cells were incubated at 37°C for 24h with vehicle control (0 µM) and different concentrations of TMCB (ab120289). Decreased expression of cIAP2 in HeLa cells correlates with an increase in TMCB concentration, as described in literature.

    Whole cell lysates were prepared with RIPA buffer (containing protease inhibitors and sodium orthovanadate), 40µg of each were loaded on the gel and the WB was run under reducing conditions. After transfer the membrane was blocked for an hour using 5% BSA before being incubated with ab23423 at 2 µg/ml and ab1801 at 2 µg/ml overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP (ab97051 ) at 1/10000 dilution and visualised using ECL development solution.

References for Anti-cIAP2 antibody (ab23423)

This product has been referenced in:
  • Puliyappadamba VT  et al. Opposing effect of EGFRWT on EGFRvIII-mediated NF-?B activation with RIP1 as a cell death switch. Cell Rep 4:764-75 (2013). Read more (PubMed: 23972990) »
  • McComb S  et al. cIAP1 and cIAP2 limit macrophage necroptosis by inhibiting Rip1 and Rip3 activation. Cell Death Differ : (2012). Read more (PubMed: 22576661) »

See all 5 Publications for this product

Product Wall

Application Flow Cytometry
Sample Human Cell (CLL patiant cells)
Permeabilization Yes - BD kit 554714
Gating Strategy live cells
Specification CLL patiant cells
Fixation BD kit 554714
Username

Mrs. Naama Gil

Verified customer

Submitted Mar 30 2016

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (vaginal epithelial cells)
Loading amount 30 µg
Specification vaginal epithelial cells
Gel Running Conditions Reduced Denaturing
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Nov 06 2012

Thank you for your reply, and we are sorry that the results have not been satisfactory.
My colleague is out of the office today, however she will get back to you as soon as she returns. Please keep us updated about the results with the other c-Rel...

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Thank you for your reply.

Many antibodies will be suitable for simultaneous incubation, however, some antibodies will interfere with each other's binding. For example, if an antibody hasa weaker binding affinity, it is often observed that inc...

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Thank you for your reply and for clarifying your protocol details.

When you say that you saw a weak band the first time you ran the gel, are you stripping and reprobing the same membrane? There will be some protein loss each time the membrane...

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Thank you for contacting us. I am sorry that these antibodies are not giving satisfactory results in your western blot. Could you please provide some additional details about your protocol?

1)Howmuch protein was loaded in the gel?We typically...

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Thank you for your enquiry. For western blot, HeLa cell lysates can be used as a positive control, as seen in our laboratory testings (datasheet WB image). Also, according to SwissProt (http://www.uniprot.org/uniprot/Q13489), the protein is hig...

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