Recombinant
RabMAb

Anti-cIAP2 antibody [E40] (ab32059)

Overview

  • Product name
    Anti-cIAP2 antibody [E40]
    See all cIAP2 primary antibodies
  • Description
    Rabbit monoclonal [E40] to cIAP2
  • Specificity
    This antibody is specific for human cIAP2. It does not cross-react with any other IAP family members.
  • Tested applications
    Suitable for: WB, IHC-P, ICC, IPmore details
    Unsuitable for: Flow Cyt
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to the residues before the breakpoint for translocation to form BIRC3-MALT1 of Human cIAP2.

  • Positive control
    • Daudi cell lysate
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Alternative versions available:

    Anti-cIAP2 antibody (HRP) [E40] (ab199501)

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab32059 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Predicted molecular weight: 72 kDa.
IHC-P Use at an assay dependent concentration.
ICC 1/100.
IP Use a concentration of 5 µg/ml.
  • Application notes
    Is unsuitable for Flow Cyt.
  • Target

    • Function
      Apoptotic suppressor. The BIR motifs region interacts with TNF receptor associated factors 1 and 2 (TRAF1 and TRAF2) to form an heteromeric complex, which is then recruited to the tumor necrosis factor receptor 2 (TNFR2).
    • Tissue specificity
      Highly expressed in fetal lung, and kidney. In the adult, expression is mainly seen in lymphoid tissues, including spleen, thymus and peripheral blood lymphocytes.
    • Involvement in disease
      Note=A chromosomal aberration involving BIRC3 is recurrent in low-grade mucosa-associated lymphoid tissue (MALT lymphoma). Translocation t(11;18)(q21;q21) with MALT1. This translocation is found in approximately 50% of cytogenetically abnormal low-grade MALT lymphoma.
    • Sequence similarities
      Belongs to the IAP family.
      Contains 3 BIR repeats.
      Contains 1 CARD domain.
      Contains 1 RING-type zinc finger.
    • Cellular localization
      Cytoplasm.
    • Information by UniProt
    • Database links
    • Alternative names
      • AIP 1 antibody
      • AIP1 antibody
      • API 2 antibody
      • API2 antibody
      • Apoptosis inhibitor 2 antibody
      • Baculoviral IAP repeat containing 3 antibody
      • Baculoviral IAP repeat containing protein 3 antibody
      • Baculoviral IAP repeat-containing protein 3 antibody
      • BIRC 3 antibody
      • BIRC3 antibody
      • BIRC3_HUMAN antibody
      • C IAP2 antibody
      • C-IAP2 antibody
      • CIAP 2 antibody
      • CIAP2 antibody
      • HAIP 1 antibody
      • HAIP1 antibody
      • HIAP 1 antibody
      • HIAP-1 antibody
      • HIAP1 antibody
      • IAP homolog C antibody
      • IAP-1 antibody
      • Inhibitor of apoptosis protein 1 antibody
      • MALT 2 antibody
      • MALT2 antibody
      • Mammalian IAP homolog C antibody
      • MIHC antibody
      • RING finger protein 49 antibody
      • RNF49 antibody
      • TNFR2 TRAF signaling complex protein 1 antibody
      • TNFR2 TRAF signalling complex protein antibody
      • TNFR2-TRAF-signaling complex protein 1 antibody
      see all

    Anti-cIAP2 antibody [E40] images

    • Anti-cIAP2 antibody [E40] (ab32059) at 1/1000 dilution + Daudi cell lysate.

      Predicted band size : 72 kDa
      Observed band size : 72 kDa
    • ab32059 at a 1:50 dilution staining cIAP2 in human normal spleen, using Immunohistochemistry, Paraffin Embedded Tissue.
    • cIAP2 was immunoprecipitated using 0.5mg Daudi whole cell extract, 5µg of Rabbit polyclonal to cIAP2 and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).
      The antibody was incubated under agitation with Protein G beads for 10min, Daudi whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.
      Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70oC; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab32059.
      Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697).
      Band: 72kDa; cIAP2

    References for Anti-cIAP2 antibody [E40] (ab32059)

    This product has been referenced in:
    • Tchoghandjian A  et al. Inhibitor of apoptosis protein expression in glioblastomas and their in vitro and in vivo targeting by SMAC mimetic GDC-0152. Cell Death Dis 7:e2325 (2016). WB ; Human . Read more (PubMed: 27490930) »
    • Alonso J  et al. NOR-1/NR4A3 regulates the cellular inhibitor of apoptosis 2 (cIAP2) in vascular cells: role in the survival response to hypoxic stress. Sci Rep 6:34056 (2016). Read more (PubMed: 27654514) »

    See all 14 Publications for this product

    Product Wall

    Application
    Western blot
    Loading amount
    20 µg
    Gel Running Conditions
    Reduced Denaturing
    Sample
    Human Cell lysate - whole cell (Epithelial)
    Specification
    Epithelial
    Treatment
    50 ng/TNF
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Username

    Abcam user community

    Verified customer

    Submitted Feb 27 2014


    Please notice, these protocols may be optimised, and could not work with your specific tissue.

    Ab32059: Steam slides in 0.01M Sodium Citrate Buffer, pH 6.0 at 125°C for 30 seconds followed by 10 seconds at 90° C into a decloaking ...

    Read More
    Application
    Western blot
    Loading amount
    25 µg
    Gel Running Conditions
    Reduced Denaturing (10% acrylamyde)
    Sample
    Human Cell lysate - whole cell (HeLa)
    Specification
    HeLa
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Username

    Abcam user community

    Verified customer

    Submitted Nov 04 2013

    Ok great, this will ship out tonight. Please keep me updated, and I look forward to hearing from you!

    Thanks for getting back to me. I'm sending a vial of ab23423 free of charge on the order ***, which we have in stock and can be delivered tomorrow. Will your receiving department be open tomorrow, or should I set it for a later delivery? Please keep me...

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    Thanks for your reply and for your patience while i have been in touch with the lab. I have heard back from the lab, and I don't see anything unusual in their protocol so I don't have a great explanation for these results. At this point, I would sugges...

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    Thank you for your reply and for sending the additional information. I am in touch with the testing laboratory to ask for further suggestions to get the correct results. I do have a few more questions, just to make sure I understand the images ...

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    Thanks for sending the image again. I'm sorry to see that the results are so poor with this replacement antibody. Do any of the bands on the blot correspond to the expected band size of cIAP2? How much lysate do you load into each well? The testing lab...

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    Thank you for keeping me updated about this situation and for sending the protocol information and image. I unfortunately can not open the Gel file. Could you re-send it in pdf or jpg? I will be happy to see if there are any suggestions that might impr...

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    Thanks for your call today and for letting us know about the trouble with these antibodies. As we discussed, I am sending a free of charge replacement vial of ab113237 on the order ***, which I expect to arrive December 1. Please keep me update...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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