Anti-CID antibody (ab10887)
Key features and details
- Rabbit polyclonal to CID
- Suitable for: WB, ICC/IF
- Reacts with: Drosophila melanogaster
- Isotype: IgG
Overview
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Product name
Anti-CID antibody -
Description
Rabbit polyclonal to CID -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IFmore details -
Species reactivity
Reacts with: Drosophila melanogaster -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- Drosophila Embryo Lysate;
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab10887 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/500 - 1/1000. Detects a band of approximately 32 kDa (predicted molecular weight: 26 kDa).
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ICC/IF |
1/100.
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Notes |
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WB
1/500 - 1/1000. Detects a band of approximately 32 kDa (predicted molecular weight: 26 kDa). |
ICC/IF
1/100. |
Target
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Function
Histone H3-like variant which exclusively replaces conventional H3 in the nucleosome core of centromeric chromatin at the inner plate of the kinetochore. Required for recruitment and assembly of kinetochore proteins, mitotic progression and chromosome segregation. May serve as an epigenetic mark that propagates centromere identity through replication and cell division. -
Sequence similarities
Belongs to the histone H3 family. -
Cellular localization
Nucleus. Chromosome, centromere, kinetochore. Localizes exclusively in the kinetochore domain of centromeres. Co-expressed with yemalpha. - Information by UniProt
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Database links
- Entrez Gene: 36495 Drosophila melanogaster
- SwissProt: Q9V6Q2 Drosophila melanogaster
- Unigene: 5691 Drosophila melanogaster
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Alternative names
- CENP A antibody
- CENP-A homolog antibody
- CENPA / CID antibody
see all
Images
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Drosophila S2 cells treated with ab10887 at 1:100 dilution and fixed with 3% PFA for 3 mins. Red = DAPI Green = ab10887
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Anti-CID antibody (ab10887) at 1 µg/ml + Drosophila embryo nuclear extract (from melanogaster embryos 0-12Hr) (ab27258) at 20 µg
Secondary
Goat polyclonal to Rabbit IgG H&L (HRP) Pre-Adsorbed at 1/10000 dilution
Performed under reducing conditions.
Predicted band size: 26 kDa
Observed band size: 26 kDa
Additional bands at: 12 kDa (possible non-specific binding), 42 kDa (possible non-specific binding)
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (13)
ab10887 has been referenced in 13 publications.
- Popova JV et al. Genetic Control of Kinetochore-Driven Microtubule Growth in Drosophila Mitosis. Cells 11:N/A (2022). PubMed: 35883570
- Bobkov GOM et al. Spt6 is a maintenance factor for centromeric CENP-A. Nat Commun 11:2919 (2020). PubMed: 32522980
- Huang A et al. Phosphorylation of Drosophila CENP-A on serine 20 regulates protein turn-over and centromere-specific loading. Nucleic Acids Res 47:10754-10770 (2019). PubMed: 31535131
- Pavlova GA et al. RNAi-mediated depletion of the NSL complex subunits leads to abnormal chromosome segregation and defective centrosome duplication in Drosophila mitosis. PLoS Genet 15:e1008371 (2019). PubMed: 31527906
- Strunov A et al. Ultrastructural analysis of mitotic Drosophila S2 cells identifies distinctive microtubule and intracellular membrane behaviors. BMC Biol 16:68 (2018). PubMed: 29907103