Overview

  • Product nameAnti-CIDE A antibody
    See all CIDE A primary antibodies
  • Description
    Rabbit polyclonal to CIDE A
  • SpecificityNo cross activity to CIDE B
  • Tested applicationsSuitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide:

    DKEERPSLRS QAKGRFT

    , corresponding to amino acids 200-217 of Human CIDE A

  • Positive control
    • Brain (Human) Tissue Lysate - adult normal tissue

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab62343 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/2000. Detects a band of approximately 23 kDa (predicted molecular weight: 25 kDa).
IHC-P Use at an assay dependent concentration.
ICC/IF Use a concentration of 20 µg/ml.

Target

  • RelevanceApoptosis is related to many diseases and induced by a family of cell death receptors and their ligands. Cell death signals are transduced by death domain containing adapter molecules and members of the caspase family of proteases. These death signals finally cause the degradation of chromosomal DNA by activated DNase. DFF45/ICARD has been identified as inhibitor of caspase activated DNase DFF40/CAD. DFF45 related proteins CIDE A and CIDE B (for cell death inducing DFF like effector A and B) were recently identified. CIDE contains a new type of domain termed CIDE N, which has high homology with the regulatory domains of DFF45/ICAD and DFF40/CAD. Expression of CIDE A induces DNA fragmentation and activates apoptosis, which is inhibited by DFF45. CIDE A is a DFF45 inhibitable effector that promotes cell death and DNA fragmentation. CIDE A is expressed in many tissues.
  • Cellular localizationNuclear. Cytoplasm; mitochondrial envelope; lipid droplet.
  • Database links
  • Alternative names
    • Cell death activator CIDE A antibody
    • Cell Death Inducing DFFA Like Effector A antibody
    • cell death inducing DNA fragmentation factor, alpha subunit like effector A antibody
    • CIDE-A antibody
    • CIDEA antibody
    see all

Anti-CIDE A antibody images

  • All lanes : Anti-CIDE A antibody (ab62343) at 1/2000 dilution

    Lane 1 : Human brain tissue lysate in the absence of the immunizing peptide
    Lane 2 : Human brain tissue lysate in the presence of the immunizing peptide


    Predicted band size : 25 kDa
    Observed band size : 23 kDa (why is the actual band size different from the predicted?)
  • ab62343 at 5ug/ml staining human brain tissue by IHC-P.
  • Immunofluorescence of CIDE-A in Human Brain cells using ab62343 at 20 ug/ml.

References for Anti-CIDE A antibody (ab62343)

ab62343 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab62343.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"