Overview

  • Product name
    Anti-Cig2 antibody [CIG 3A11/5]
  • Description
    Mouse monoclonal [CIG 3A11/5] to Cig2
  • Host species
    Mouse
  • Tested applications
    Suitable for: WB, ELISA, IPmore details
  • Species reactivity
    Reacts with: Schizosaccharomyces pombe
  • Immunogen

    Bacterial S. pombe Cig2 purified protein.

Properties

Applications

Our Abpromise guarantee covers the use of ab10881 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000.
ELISA Use at an assay dependent dilution.
IP Use at an assay dependent dilution.

Target

  • Relevance
    Cig2 is a fission yeast (Schizosaccharomyces pombe) B-type S-phase cyclin and is expressed in a sharp spike peaking at G1/S period. Cig2 binds to Cdc2 (CDK), and the resulting Cdc2-Cig2 complex controls the G1/S transition of the cell cycle.
  • Alternative names
    • B type S phase cyclin antibody

Images

  • Western blot - Anti-Cig2 antibody [CIG 3A11/5] (ab10881)
    Western blot - Anti-Cig2 antibody [CIG 3A11/5] (ab10881)This image is courtesy of an anonymous Abreview
    All lanes : Anti-Cig2 antibody [CIG 3A11/5] (ab10881) at 1/1000 dilution

    Lane 1 : 20ug untreated S. pombe wildtype cells, whole cell lysate
    Lane 2 : 20ug S. pombe wildtype cells 8mM HU treated for 1 hour, whole cell lysate
    Lane 3 : 20ug S. pombe wildtype cells 8mM HU treated for 2 hours, whole cell lysate
    Lane 4 : 20ug S. pombe wildtype cells 8mM HU treated for 3 hours, whole cell lysate

    Secondary
    All lanes : HRP conjugated sheep anti-mouse antibody

    Developed using the ECL technique.

    Observed band size: 50 kDa (why is the actual band size different from the predicted?)


    Exposure time: 1 minute

    See Abreview

References

This product has been referenced in:
  • Chu Z  et al. A Cds1-mediated checkpoint protects the MBF activator Rep2 from ubiquitination by anaphase-promoting complex/cyclosome-Ste9 at S-phase arrest in fission yeast. Mol Cell Biol 29:4959-70 (2009). Read more (PubMed: 19596787) »
  • Chu Z  et al. Modulation of cell cycle-specific gene expressions at the onset of S phase arrest contributes to the robust DNA replication checkpoint response in fission yeast. Mol Biol Cell 18:1756-67 (2007). Read more (PubMed: 17332498) »

See all 3 Publications for this product

Publishing research using ab10881? Please let us know so that we can cite the reference in this datasheet.

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Western blot abreview for Anti-Cig2 antibody [CIG 3A11/5]

 Excellent
Abreviews
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Schizosaccharomyces pombe Cell lysate - whole cell (S. pombe wildtype cells)
Loading amount
20 µg
Specification
S. pombe wildtype cells
Treatment
8mM HU treated for 0, 1, 2 and 3 hours
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% milk
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Submitted Sep 11 2006

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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